Characteristics of saxitoxin binding to the sodium channel of sarcolemma isolated from rat skeletal muscle

The Journal of Physiology
R L Barchi, J B Weigele

Abstract

1. The characteristics of saxitoxin (STX) binding to the mammalian Na channel have been studied in purified sarcolemma isolated from rat skeletal muscle. 2. STX binds specifically to isolated sarcolemma with a Kd of 1.43 x 10(-9) M and Bmax of 7-8 p-mole STX bound/mg membrane protein at 0 degrees C in the presence of 140 mM-NaCl. In rat muscle homogenate under the same conditions the corresponding values are Kd = 1.53 x 10(-9) M and Bmax = 0.15-0.20 p-mole/mg protein (18-20 p-mole/g wet wt.). Membrane purification produced a fortyfold increase in STX binding site concentration per milligram protein. Calculated binding site density in isolated sarcolemma was about 30 sites/micron 2 of membrane surface. 3. Denervation (10-14 days) results in a 43% reduction in the density of high-affinity STX binding sites in purified sarcolemma, but the Kd for this class of sites is not changed. 4. In sarcolemma, the apparent Kd for STX binding is dependent on temperature pH and ionic strength. The Q10 for Kd between 0 and 40 degrees C is 1.3. Protonation of a group having a pK of 6.0 markedly raises Kd without affecting Bmax. Apparent Kd increases eightfold when ionic strength is raised from 20 to 600 mM. 5. Dissociation and association rate co...Continue Reading

References

Dec 1, 1991·Pflügers Archiv : European journal of physiology·G Hansen, W Ulbricht
Aug 1, 1990·Pflügers Archiv : European journal of physiology·U LönnendonkerR Stämpfli
Jan 1, 1985·Progress in Neurobiology·D Yamamoto
Sep 9, 2000·Toxicon : Official Journal of the International Society on Toxinology·Lyndon E Llewellyn, J Doyle
Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·P VélezB A Suárez-Isla
Jun 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·G StrichartzJ A Debin
Nov 1, 1994·Journal of Pharmacological and Toxicological Methods·L Bambrick, T Gordon
Jan 1, 1993·Molecular Neurobiology·R G KallenR L Barchi
Jan 1, 1989·Molecular Neurobiology·R Laufer, J P Changeux
Jan 1, 1986·Membrane Biochemistry·A Uehara, Edward G Moczydlowski
Apr 9, 2005·IEEE Transactions on Nanobioscience·Robert J French, Gerald W Zamponi
Nov 1, 1985·Experimental Neurology·J E MollmanR L Barchi
Oct 1, 1981·European Journal of Biochemistry·C FrelinM Lazdunski
Jan 1, 1986·Annals of the New York Academy of Sciences·R B Rogart
Jan 1, 1986·Annals of the New York Academy of Sciences·Edward G MoczydlowskiJ Heiny
Jan 1, 1986·Annals of the New York Academy of Sciences·G StrichartzC H Bay
Jan 1, 1986·Annals of the New York Academy of Sciences·W UlbrichtJ Schmidtmayer
Dec 25, 2012·Toxicon : Official Journal of the International Society on Toxinology·Edward G Moczydlowski
Jan 1, 1985·Pflügers Archiv : European journal of physiology·K M Gülden, W Vogel
Mar 1, 1984·Pflügers Archiv : European journal of physiology·B A KotsiasP Horowicz
Mar 31, 2006·Natural Product Reports·Lyndon E Llewellyn
Apr 29, 2014·Angewandte Chemie·Arun P ThottumkaraJ Du Bois
Feb 16, 2006·The Journal of Biological Chemistry·Inna N RybakovaJames M Ervasti
May 1, 1980·European Journal of Biochemistry·Y JacquesM Lazdunski

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