Characteristics of Skeletal Muscle Fibers of SOD1 Knockout Mice

Oxidative Medicine and Cellular Longevity
Hiroshi NagahisaHirofumi Miyata

Abstract

Cu/Zn superoxide dismutase (SOD1) knockout (KO) mice are known as an aging model in some aspects, but the damage and regeneration process of each fiber type have not been sufficiently studied. In this study, we investigated the damage and satellite cell state of the gastrocnemius muscle in SOD1 KO mice (6 months old) using immunohistochemical staining and real-time RT-PCR. The proportion of central nuclei-containing Type IIx/b fibers in the deep and superficial portions of the gastrocnemius muscle was significantly higher in SOD1 KO than control mice. The number of satellite cells per muscle fiber decreased in all muscle fiber types in the deep portion of the gastrocnemius muscle in SOD1 KO mice. In addition, the mRNA expression levels of Pax7 and myogenin, which are expressed in satellite cells in the activation, proliferation, and differentiation states, significantly increased in the gastrocnemius muscle of SOD1 KO mice. Furthermore, mRNA of myosin heavy chain-embryonic, which is expressed in the early phase of muscle regeneration, significantly increased in SOD1 KO mice. It was suggested that muscle is damaged by reactive oxygen species produced in the mitochondrial intermembrane space in Type IIxb fibers, accelerating the ...Continue Reading

References

Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·N S ChandelP T Schumacker
Dec 31, 2003·Trends in Genetics : TIG·Matthias Ernst, Brendan J Jenkins
Jan 16, 2004·Experimental Gerontology·Stefania FulleGiorgio Fanò
Oct 28, 2005·American Journal of Physiology. Cell Physiology·Ethan J Anderson, P Darrell Neufer
Dec 27, 2005·Respiratory Physiology & Neurobiology·Leonardo F FerreiraDavid C Poole
Nov 17, 2006·Cell and Tissue Research·Tatiana Y KostrominovaJohn A Faulkner
Jan 8, 2008·Cell Metabolism·Antonio L SerranoPura Muñoz-Cánoves
Dec 17, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jorge L Gamboa, Francisco H Andrade
Dec 31, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Youngmok C JangHolly Van Remmen
Dec 3, 2010·American Journal of Physiology. Cell Physiology·William W Wheaton, Navdeep S Chandel
Oct 2, 2012·Nature·Joe V ChakkalakalAndrew S Brack
Jul 16, 2013·Free Radical Biology & Medicine·Aphrodite Vasilaki, Malcolm J Jackson
Sep 7, 2013·Biomolecules & Therapeutics·Dong Hoon Kang, Sang Won Kang

❮ Previous
Next ❯

Citations

Mar 10, 2019·Neurology·Leonard H van den BergUNKNOWN Airlie House ALS Clinical Trials Guidelines Group
Jul 28, 2018·Oxidative Medicine and Cellular Longevity·Takujiro HommaJunichi Fujii
Nov 9, 2018·Pain Research & Management : the Journal of the Canadian Pain Society = Journal De La Société Canadienne Pour Le Traitement De La Douleur·Hiroshi NagahisaHirofumi Miyata

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Adobe Photoshop
Primer Express

Related Concepts

Related Feeds

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.