PMID: 2877909Nov 1, 1986

Characteristics of the beta-adrenergic receptor in the rat ventral prostate using [125I]cyanopindolol

Molecular and Cellular Endocrinology
P PoyetF Labrie

Abstract

The binding characteristics of the beta-adrenergic receptor in the rat ventral prostate homogenate have been studied using the highly potent beta-adrenergic antagonist [125I]cyanopindolol (CYP) as ligand. The bound ligand was separated from the free moiety by precipitation with polyethylene glycol (PEG-6000). This technique is simple, accurate, fast and more advantageous than filtration of the hormone-receptor complex on glass fiber filters or direct centrifugation. [125I]CYP binds to a single class of high affinity sites at an apparent KD value of 23 pM. Using 0.1 microM (-)propranolol to determine non-specific binding, a number of sites of 600 fmol/mg protein were measured. The observed order of potency of adrenergic agonists (KD values) in competing for [125I]CYP binding was: (-)isoproterenol (25 nM) greater than (-)epinephrine (74 nM) much greater than (-)norepinephrine (1900 nM). Detailed study of the binding potency of a large series of beta 1- and beta 2-adrenergic agonists and antagonists showed the presence of a typical beta 2-subtype adrenergic receptor in the rat ventral prostate. The best estimate indicates that the proportion of beta 2-adrenergic receptors in rat ventral prostate is more than 95% of the total popul...Continue Reading

References

Jun 1, 1988·Journal of Steroid Biochemistry·B Marchetti, F Labrie
Jun 1, 1992·Journal of Neuroendocrinology·R J Heiliwell, L M Williams
Feb 9, 2006·British Journal of Pharmacology·Martin C Michel, Wim Vrydag
Feb 1, 1991·Molecular and Cellular Neurosciences·M G MartinoliG Pelletier
Mar 16, 2019·Cancers·George Kulik

Citations

May 1, 1976·Journal of Medicinal Chemistry·D L TempleL E Allen
Mar 1, 1975·Biochemical Pharmacology·R J Lefkowitz
Nov 18, 1978·The Veterinary Record·R J Thomas, J R Starr
Dec 30, 1971·Annals of the New York Academy of Sciences·R L Singhal
Nov 1, 1972·British Journal of Pharmacology·M C GweeI W Rodger
Feb 1, 1973·Life Sciences. Pt. 1: Physiology and Pharmacology·B AbladL Ek
Jan 1, 1968·British Journal of Pharmacology and Chemotherapy·D Dunlop, R G Shanks
Feb 23, 1972·Nature: New Biology·R A Burges, K J Blackburn
Feb 8, 1973·Life Sciences. Pt. 2: Biochemistry, General and Molecular Biology·W Schwarzlose, F Heim
Nov 1, 1967·Life Sciences·A M LandsH J Buzzo
Jan 1, 1982·The Prostate·C J JesikC Lee
May 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·S ShimaM Asakura

Related Concepts

Visken
Filtration
August Rats
Catecholamines Measurement
Adrenergic Agonists
Norepinephrine, (+, -)-Isomer
Centrifugation
ADRB2 gene
Prostate
Epinephrine Measurement

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.