PMID: 8596721Mar 1, 1996Paper

Characteristics of the delayed rectifier K current compared in myocytes isolated from the atrioventricular node and ventricle of the rabbit heart

Pflügers Archiv : European journal of physiology
F C HowarthJules C Hancox

Abstract

The delayed rectifier potassium current (IK) is known to be important in action potential repolarisation and may contribute to the diastolic pacemaker depolarisation in pacemaker cells from the heart. In this study, using whole-cell patch clamp, we investigated the characteristics of IK in morphologically normal cells from the atrioventricular node (AVN) and ventricle of the rabbit heart. Cells were held at -40 mV and 5 microM external nifedipine was used to block L-type calcium current (ICa,L). Significant IK was observed with pulses to potentials more positive than -30 mV. The steady-state activation curve in both cell types showed maximal activation at between + 10 and + 20 mV. Half-maximal activation of IK occurred at -4.9 and -4.1 mV with slope factors of 8.3 and 12.4 mV in ventricular and AVN cells, respectively. Using pulses of increasing duration, significant IK tails after repolarisation from + 40 mV were observed with pulses of 20 ms and increased with pulses up to 100-120 ms in both cell types. Pulses of longer duration did not activate further IK and this suggested that only the rapid component of IK, called IKr, was present in either cell type. Moreover, IK tails after pulses to all potentials were blocked complete...Continue Reading

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Citations

Jun 27, 2007·International Heart Journal·Ayumi YokoyamaKenjiro Kikuchi
Jul 20, 2000·Journal of Molecular and Cellular Cardiology·A J WorkmanA C Rankin
Nov 19, 2009·International Journal of Cardiology·Arie O VerkerkRuben Coronel
Oct 28, 2008·Journal of Molecular and Cellular Cardiology·Hongwei ChengJules C Hancox
Jun 29, 2007·Journal of Pharmacological and Toxicological Methods·Derek A TerrarB M Heath
Nov 6, 2004·Neuropharmacology·Zoe A McCrossan, Geoffrey W Abbott
Aug 27, 2014·Journal of Cardiovascular Electrophysiology·Rosanne VarkevisserMarcel A VAN DER Heyden
Jun 15, 2007·Biochemical Pharmacology·R S DuncanJ C Hancox
Sep 16, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Igor R EfimovMark Boyett
Jun 1, 1997·Journal of Cardiovascular Electrophysiology·A J LeviJ V Jones
Sep 27, 2005·Physiological Reviews·Jeanne M Nerbonne, Robert S Kass
Aug 30, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Xun WangAmber L Pond
Sep 29, 2011·British Journal of Pharmacology·Hongwei ChengJules C Hancox
Jul 22, 2017·Molecular Medicine Reports·Frank Christopher HowarthThomas Edward Adrian

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