Abstract
The interferon regulatory factor (IRF) family plays critical roles in a host's virus infection responses. In this study, two IRF family members, zfIRF5 and zfIRF7, are identified in zebrafish. The zfIRF5 protein encodes 297 amino acids without the carboxyl IRF3 domain. We suggest that zfIRF5 is a new kind of splicing variant, following the nine other kinds of IRF5 splicing variants found in mammals. The zfIRF7 protein is identified as a member of the IRF7 family, compared to the human IRF7 protein, the amino acid sequence of zfIRF7 only with 29% identity and devoid a virus activated domain (VAD). There zfIRF5/7 proteins are expressed in all 11 selective zebrafish tissues within 6-120h of embryonic development. Laser confocal microscopy shows that the full length the proteins are separately located in the cytoplasm. Mutation experiments show that the nuclear localization signals (NLS) of zfIRF7 and zfIRF-5 are at the N-terminal and C-terminals, respectively. In the assays, zfIRF7 expression increases during infectious spleen and kidney necrosis virus (ISKNV) infection and by poly(I:C) and LPS injections, both of which activate the transcriptional activity of L8G5-luc plasmids. The over-expression of zfIRF5/7 activates the interf...Continue Reading
References
Dec 29, 1995·Cell·D Thanos, T Maniatis
Oct 24, 1998·The Journal of Biological Chemistry·W C AuP M Pitha
Nov 21, 1998·The EMBO Journal·I MariéD E Levy
Jan 7, 1999·FEBS Letters·M SatoN Tanaka
Jan 8, 2000·Immunity·W M ChuM Karin
Nov 9, 2000·Immunity·M SatoT Taniguchi
Feb 13, 2001·Virology·W C AuP M Pitha
Mar 30, 2001·The Journal of Biological Chemistry·R XieG S Stein
Jul 27, 2001·The Journal of Biological Chemistry·W C Au, P M Pitha
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·I G Macara
Feb 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Betsy BarnesPaula M Pitha
Feb 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Mitsutoshi YoneyamaTakashi Fujita
Feb 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Nancy C Reich
Jul 26, 2002·Molecular and Cellular Biology·Betsy J BarnesPaula M Pitha
Feb 26, 2003·The Journal of Biological Chemistry·Betsy J BarnesPaula M Pitha-Rowe
Apr 19, 2003·Science·Sonia SharmaJohn Hiscott
Jan 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paula M Pitha
Nov 24, 2004·The Journal of Biological Chemistry·Rongtuan LinJohn Hiscott
Jan 25, 2005·Immunity·Yanxing YuGary S Hayward
Jan 25, 2005·Nature·Akinori TakaokaTadatsugu Taniguchi
Feb 8, 2005·The Journal of Biological Chemistry·Annett SchoenemeyerDouglas T Golenbock
Apr 1, 2005·Nature·Kenya HondaTadatsugu Taniguchi
Apr 5, 2005·The Journal of Biological Chemistry·Margo E ManclBetsy J Barnes
Jul 4, 2006·Biochemical and Biophysical Research Communications·Zhiming XiangYongqing Li
Aug 26, 2006·Nature Reviews. Immunology·Kenya Honda, Tadatsugu Taniguchi
Nov 28, 2007·Genome Biology·Cornelia SteinMaria Leptin
Apr 22, 2008·Virology·Xiaopeng XuJianguo He
May 20, 2008·Virus Research·Chuanfu DongJianguo He
Citations
Mar 4, 2015·Fish & Shellfish Immunology·Sabrina M InkpenMatthew L Rise
Jun 23, 2012·Developmental and Comparative Immunology·Yi-Bing Zhang, Jian-Fang Gui
Jun 16, 2012·Developmental and Comparative Immunology·Guo-Bin HuShi-Cui Zhang
Nov 9, 2011·Fish & Shellfish Immunology·Jun XiaShi-Cui Zhang
Apr 30, 2011·Developmental and Comparative Immunology·Zhiming XiangJianguo He
Oct 12, 2013·Journal of Fish Diseases·G W ShinJ H Kim
Feb 25, 2014·Fish & Shellfish Immunology·W D Niroshana WickramaarachchiIlson Whang
Apr 12, 2015·Fish & Shellfish Immunology·Lin Qi, Zhiming Xiang
Mar 12, 2015·Journal of Immunology Research·Youliang Rao, Jianguo Su
Jun 8, 2013·Gene·Zhao-Yu LiuJian-Guo He
Mar 4, 2015·Fish & Shellfish Immunology·Jian ZhangYong-Hua Hu
May 29, 2014·Trends in Microbiology·Jean-Pierre LevraudPierre Boudinot
May 20, 2014·Gene·Zhiming XiangChuangfu Dong
Oct 31, 2014·Journal of Virology·Ji-Min YuanJian-Guo He
Jun 29, 2016·BMC Veterinary Research·Yaoyao ZhuGuiwen Yang
Dec 7, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Long-Feng LuYong-An Zhang
Nov 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Iliana SerifiThomais Papamarcaki
Aug 16, 2019·Journal of Virology·Long-Feng LuShun Li
Jan 23, 2021·Developmental and Comparative Immunology·Fan-Xiang GaoZhe Zhao
Jan 29, 2020·Ecotoxicology and Environmental Safety·Sheng ChenChunsheng Liu