Characteristics of the tree shrew gut virome

PloS One
Linxia ChenJie-Jie Dai

Abstract

The tree shrew (Tupaia belangeri) has been proposed as an alternative laboratory animal to primates in biomedical research in recent years. However, characteristics of the tree shrew gut virome remain unclear. In this study, the metagenomic analysis method was used to identify the features of gut virome from fecal samples of this animal. Results showed that 5.80% of sequence reads in the libraries exhibited significant similarity to sequences deposited in the viral reference database (NCBI non-redundant nucleotide databases, viral protein databases and ACLAME database), and these reads were further classified into three major orders: Caudovirales (58.0%), Picornavirales (16.0%), and Herpesvirales (6.0%). Siphoviridae (46.0%), Myoviridae (45.0%), and Podoviridae (8.0%) comprised most Caudovirales. Picornaviridae (99.9%) and Herpesviridae (99.0%) were the primary families of Picornavirales and Herpesvirales, respectively. According to the host types and nucleic acid classifications, all of the related viruses in this study were divided into bacterial phage (61.83%), animal-specific virus (34.50%), plant-specific virus (0.09%), insect-specific virus (0.08%) and other viruses (3.50%). The dsDNA virus accounted for 51.13% of the tot...Continue Reading

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Datasets Mentioned

BETA
SRP154022
ALAR00000000
AF258784.1
FJ483968
X56019
U32924
M20562
KM516909

Methods Mentioned

BETA
PCR
Illumina

Software Mentioned

bowtie2
BLASTx
MEGA
CLC Genomics Workbench
Clone Manager Professional Suite
tBLASTx
Lasergene
BLASTn
DNAStar
BLAST

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