PMID: 9419011Jan 7, 1998Paper

Characteristics of thrombin-induced calcium signals in rat astrocytes

Glia
Joachim J Ubl, G Reiser

Abstract

The protease thrombin seems to play a central role in events following neural injury, whereby the enzyme can act, in concert with other molecules as a hormone or as a growth factor. In cells derived from the nervous system, thrombin induces changes in morphology and proliferation. The signalling mechanisms involved in these thrombin-activated processes are still unclear. In the present study we investigated Ca2+ signals in fura-2 loaded rat astrocytes in primary culture. Brief stimulation of astrocytes with thrombin induced a dose-dependent transient elevation of [Ca2+]i, best fitted by a double-sigmoidal curve giving two EC50 values of 3 pM and 150 pM. Continuous superfusion of cells with thrombin induced Ca2+ responses with three different types of kinetics. In 48% of the cells tested a single transient rise superimposed with fast fluctuations of [Ca2+]i was seen. The following complex long-term changes of [Ca2+]i, dependent on the presence of the agonist thrombin, were observed: i) a biphasic [Ca2+]i elevation, characterized by an initial peak followed by a sustained plateau phase (in 43% of the cells) and ii) oscillations of [Ca2+]i (in 9% of the cells). The observed Ca2+ responses were inhibited by the phospholipase C (PLC...Continue Reading

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Citations

Sep 26, 2000·Cytometry·M C CatlinL G Costa
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·V B MahajanD D Cunningham
Jan 6, 2015·Stem Cell Reports·Massimiliano CaiazzoVania Broccoli
Jul 28, 2006·Chembiochem : a European Journal of Chemical Biology·Einat SharonBilha Fischer
Jan 15, 2014·Toxicon : Official Journal of the International Society on Toxinology·Camila M YonamineMaria Teresa R Lima-Landman
Mar 27, 2004·Physiological Reviews·Valeria S Ossovskaya, Nigel W Bunnett
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Hong WangGeorg Reiser
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Jun 20, 2001·The Journal of Biological Chemistry·K S PaiD D Cunningham
Sep 15, 2000·Journal of Neurochemistry·T MöllerB R Ransom

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