PMID: 3752164Oct 1, 1986Paper

Characteristics of thyroxine 5'-deiodinase activity in human liver

The American Journal of the Medical Sciences
J J HardyR D Utiger

Abstract

The conversion of thyroxine (T4) to triiodothyronine (T3) was studied in homogenates and subcellular fractions of 10 human liver specimens obtained postmortem. Preliminary studies indicated that T4 5'-deiodinase activity did not decline in rat liver kept at 5 degrees C for 6 and 24 hr after death. All human liver homogenates but one catalyzed T3 production, although the quantity of T3 produced varied greatly, from 8-fold in the absence of dithiothreitol (DTT) to 100-fold in its presence. The wide variation in activity found may reflect either postmortem loss or premortem decline in enzyme activity due to unrecognized nonthyroidal illness. The amount of T3 produced was dependent on substrate availability, protein concentration, time, pH and temperature, and enzyme activity was greatest in the microsomal fraction. T3 production was stimulated by DTT and inhibited by propylthiouracil (PTU). Thus, human liver T4 5'-deiodinase has properties very similar to the same enzyme in rat liver. These data suggest that results of studies of the effects of nonthyroidal illnesses and drugs on T4 5'-deiodinase activity in rat liver may be extrapolated to humans.

Citations

Aug 1, 1988·Biological Psychiatry·C M BeasleyD L Garver
Nov 8, 2018·Endocrine-related Cancer·Iuri Martin GoemannAna Luiza Maia
Apr 28, 2017·Journal of Molecular Microbiology and Biotechnology·Milton H Saier, J T Trevors
Dec 1, 1989·Metabolism: Clinical and Experimental·J K LeeM M Kaplan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.