Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors

Scientific Reports
Xueli BaiTingbo Liang

Abstract

Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important imm...Continue Reading

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Citations

Jan 14, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yupeng Hong, Qi Zhang
Jun 15, 2016·BMC Genomics·Dmitriy V BagaevMikhail Shugay
Jul 28, 2016·Scientific Reports·Eric LevyOlivier Harismendy
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Nov 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·John F BeausangStephen R Quake
Jul 17, 2020·The Journal of Clinical Endocrinology and Metabolism·Xiabin LanMinghua Ge
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Jul 12, 2017·BMC Biotechnology·Elisa RosatiAndre Franke
Dec 4, 2020·Journal for Immunotherapy of Cancer·Siwen Hu-LieskovanHolden T Maecker
Apr 7, 2021·Human Vaccines & Immunotherapeutics·Pingsen ZhaoXianzhu Xia
Nov 23, 2021·The Journal of Experimental Medicine·Chuan LiBrian R Gastman

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Methods Mentioned

BETA
single cell sequencing
PCR

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