Characteristics of unbuffered gel-immobilized urease particles. I. Internal pH

Biotechnology and Bioengineering
B AtkinsonI Rousseau

Abstract

The overall rate of reaction of a gel-immobilized urease particle necessarily depends upon the hydrogen ion concentrations within the particle. When the particle is unbuffered, the internal hydrogen ion concentrations are a consequence of the local rates of reaction and the rate of egress of the products of hydrolysis. A simple apparatus has been devised which allows a fairly rapid determination of the hydrogen ion concentration in the center of a particle for any given size, enzyme concentration, substrate concentration, and external pH. The products of urea hydrolysis are self-buffering in the region of pH 8.83 and for an external pH less than the self-buffering pH, the pH within the particle is increased because of the reaction. When the external pH is greater than the self-buffering pH, the converse occurs. The pH at the center of the particle approaches the self-buffering pH with an increase in particle size and enzyme concentration. The external increase in the external substrate concentration has a limited effect, simply rendering the local rates of reaction to be of zero order. The center-line pH and therefore all internal hydrogen ion concentrations depend upon the parameter L square root pe and the external pH. Differ...Continue Reading

References

Oct 1, 1974·Biotechnology and Bioengineering·J E Bailey, M T Chow
Sep 14, 1973·Biochimica Et Biophysica Acta·H NilssonK Mosbach
Apr 23, 1974·Biochemical and Biophysical Research Communications·F X HasselbergerR W Coughlin
Sep 12, 1967·Biochimica Et Biophysica Acta·K R Lynn

Citations

Sep 5, 2002·Biotechnology and Bioengineering·Douglas S Clark, J E Bailey
Jul 1, 1977·Biotechnology and Bioengineering·B Atkinson, I Rousseau
Aug 1, 1978·Biotechnology and Bioengineering·S Y YeungJ E Bailey
Sep 23, 2011·Applied Biochemistry and Biotechnology·Neelam MishraAnita Bahadur
Dec 7, 1979·Biochimica Et Biophysica Acta·D Vallin, C Tran-Minh
Nov 1, 1983·Biophysical Chemistry·J C VincentE Selegny

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