Abstract
OX40, a membrane-bound molecule of the tumor-necrosis-factor-receptor superfamily, is a critical costimulatory receptor during the immune response. Here, we newly generated two specific mouse antihuman OX40 monoclonal antibodies (mAbs) (2G2 and 1F7), whose specificities are quite different from the available OX40 mAb (ACT35) by competition assay. It was also found that both mAbs could enhance the proliferation, activation and differentiation of T lymphocytes primed by anti-CD3 mAb. These results evidenced that both were functional antihuman OX40 mAbs. Furthermore, stained by 2G2 and 1F7, FCM and immunohistochemistry detected the constitutive expression of OX40 on tumor cell lines from epithelium, breast cancer and glioma tissues. Meanwhile, the non-tumor tissues (thyroid gland, stomach gland) were also found OX40 expression. These results suggested that OX40 is not only expressed in activated T cells, but also in some tumors as well as normal gland tissues. Such expression pattern indicated that OX40 may be a valuable surface antigen in unveiling its expression and function outside the immune system. Briefly, these novel antibodies may contribute to the evaluation of the mechanism of tumor metastasis and eventually shed light o...Continue Reading
References
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Mar 1, 1994·European Journal of Immunology·U LatzaH Stein
Apr 1, 1995·European Journal of Immunology·M L BirkelandA N Barclay
Aug 1, 1996·European Journal of Immunology·A al-ShamkhaniA N Barclay
Jul 3, 1998·British Journal of Rheumatology·D BrugnoniR Cattaneo
Oct 29, 1998·Biochemical and Biophysical Research Communications·H AkibaK Okumura
Jan 8, 2000·Immunity·M KopfM F Bachmann
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A D WeinbergJ Shields
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I GramagliaM Croft
Mar 7, 2001·Cellular and Molecular Life Sciences : CMLS·U Schönbeck, P Libby
Aug 2, 2001·Nature Medicine·P Bansal-PakalaM Croft
Aug 24, 2001·Breast Cancer Research and Treatment·A MorrisA D Weinberg
Sep 25, 2001·Immunity·P R RogersM Croft
Apr 4, 2002·Trends in Immunology·Andrew D Weinberg
Dec 19, 2002·Cancer Gene Therapy·Alex W Tong, Marvin J Stone
Dec 4, 2003·Journal of Neuroimmunology·Susanna CarboniClaudia Peña-Rossi
Jun 3, 2004·Nature Reviews. Immunology·Kazuo SugamuraAndrew D Weinberg
Oct 22, 2004·Tissue Antigens·Q WangX Zhang
Jul 5, 2005·Cancer Research·Yu-Tzu TaiKenneth C Anderson
Jul 21, 2005·Tissue Antigens·G-B ZhangX-G Zhang
Sep 17, 2005·Cellular Immunology·Zhong-Wen SunXue-guang Zhang
Sep 17, 2005·Cancer Research·Che-Leung LawIqbal S Grewal
Citations
Feb 26, 2009·Hybridoma·Zhongqin ChengXueguang Zhang
Jun 24, 2010·Hybridoma·Jiamin WangXueguang Zhang
Jul 17, 2010·Pathology, Research and Practice·Fang XieXueguang Zhang
Nov 10, 2007·Tissue Antigens·G-B ZhangX-G Zhang
Mar 23, 2010·Journal of Neuroimmunology·Fang XieXueguang Zhang
Jul 31, 2013·Analytical Chemistry·Antoine FleitzFa-Yun Che
May 8, 2019·Cancers·Sebastian ChrétienTheodoros Foukakis
Nov 10, 2017·Blood·Sarah L BuchanAymen Al-Shamkhani
Sep 10, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kui S VooYong J Liu
Oct 8, 2011·International Journal of Immunopathology and Pharmacology·Q ShiYu Hua Qiu
Jan 13, 2018·Cancer Immunology Research·Tina NueblingHelmut Rainer Salih
Jan 12, 2021·Journal for Immunotherapy of Cancer·Jordana GriffithsMark S Cragg
Jul 9, 2021·JCI Insight·Toshihito HiraiSanjiv S Gambhir