PMID: 6413784Sep 1, 1983Paper

Characterization and assay of progesterone-binding components in DMBA-induced rat mammary carcinoma tissue after progesterone administration

Journal of Steroid Biochemistry
A GeierB Lunenfeld

Abstract

Nuclear and cytoplasmic progesterone-binding components were characterized and measured in DMBA-induced rat mammary carcinoma tissue, before and after progesterone administration. Rats, bearing growing tumors, were ovariectomized and then primed for two days with estradiol. Biopsy specimens were taken prior to or following administration of progesterone. Nuclear binding was assayed in the 0.4 M KCl extract of the nuclear fraction using [3H]R5020 as ligand. The receptor character of the binding was demonstrated by: (1) high affinity (Kd approximately 2 nM); (2) specificity: competition by R5020 and progesterone, minimal competition by 17 alpha-hydroxyprogesterone, corticosterone, testosterone and estradiol; (3) sedimentation constant at about 3S in a sucrose density gradient. Similar characteristics displayed the cytoplasmic receptor before and after progesterone administration. Progesterone receptor distribution in the nuclear extract and cytosol were determined in 36 tumors. The levels of total receptors (cytoplasmic plus nuclear) before and after progesterone administration varied widely, however the average values found after progesterone administration were significantly lower, 1.59 +/- 0.20 pmol/mg DNA compared to 2.58 +/-...Continue Reading

References

Mar 1, 1975·Journal of Steroid Biochemistry·I S Edelman
Jan 1, 1976·Annual Review of Physiology·J Gorski, F Gannon
Mar 1, 1979·The Journal of Endocrinology·A GeierB Lunenfeld
Jul 1, 1979·The Journal of Clinical Endocrinology and Metabolism·G Giannopoulos, D Tulchinsky
Jan 1, 1978·The Journal of Endocrinology·M T Vu Hai, E Milgrom
Nov 1, 1976·Journal of Steroid Biochemistry·B W O'MalleyW T Schrader
Jan 1, 1972·Annual Review of Biochemistry·E V Jensen, E R DeSombre
Jan 1, 1980·Endocrine Reviews·T G Muldoon
Mar 1, 1980·The Journal of Clinical Endocrinology and Metabolism·A GeierB Lunenfeld
Jan 1, 1996·Breast Cancer Research and Treatment·M A FanelliD R Ciocca
Mar 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·C HUGGINSS MORII

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