PMID: 8938575Nov 1, 1996Paper

Characterization and biologic activities of recombinant rat soluble interleukin-6 receptor

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
V ThibaultJ Gauldie

Abstract

The soluble interleukin-6 receptor (sIL-6R) consists of the extracellular domain of the membrane-bound IL-6 receptor (gp80) found on many types of cells. Contrary to most other soluble cytokine receptors, it possesses in vitro agonistic properties, yet its physiologic role remains unknown. We have generated a cDNA encoding the rat sIL-6R and have expressed and purified the protein using Escherichia coli and baculovirus systems. Analysis of purified protein by electrophoresis and silver staining showed a single band migrating at 35 kDa for E. coli (nonglycosylated) and at 47 kDa for baculovirus-derived material. The purified protein is biologically active, as determined by the ability to convert human hepatoma cells (HepG2) from nonresponsive to responsive to rat IL-6 and induce acute-phase protein synthesis. Most important, we show that rat sIL-6R directly induces proliferation of the IL-6-dependent murine hybridoma cell line (B9) in an IL-6-like manner, with 50% proliferation induced by 100 ng/ml of baculovirus-derived receptor protein. Physiologic concentrations of sIL-6R dramatically enhance the sensitivity of B9 cells to IL-6, indicating that the bioassay for IL-6 is susceptible to modulation by the presence of sIL-6R in ro...Continue Reading

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Citations

Mar 21, 2000·Journal of Neuroscience Research·M ThierS Rose-John
Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·A N von BethmannS Uhlig
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·W Y ParkT R Martin
Jul 11, 2000·The European Journal of Neuroscience·P G MurphyP M Richardson
Mar 24, 2021·Angewandte Chemie·Andreas ReifCarlo Unverzagt

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