Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro

PloS One
Shifa YangRuiliang Zhu

Abstract

Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator. However, the components of TPPPS have not been recognized. To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation. Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography. The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively. Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2. TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion. By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity. TPPPS3 at 50 μg/mL si...Continue Reading

References

Feb 11, 1992·European Journal of Pharmacology·A IalentiM Di Rosa
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M S MulliganP A Ward
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·V L DawsonS H Snyder
Mar 7, 2000·Current Medicinal Chemistry·V E Ooi, F Liu
Aug 11, 2000·Chest·C A Dinarello
Jul 20, 2002·Biologicals : Journal of the International Association of Biological Standardization·Chi Jen Lee
May 1, 2004·Journal of Medicinal Food·Tae-Soo LimJae-Kwan Hwang
Sep 27, 2005·Journal of Ethnopharmacology·Kun Yeong LeeYoung Jin Jeon
Jan 24, 2006·International Immunopharmacology·Igor A Schepetkin, Mark T Quinn
May 20, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Howard L Kaufman, Jedd D Wolchok
Jul 15, 2006·International Journal of Biological Macromolecules·Tiehong YangQibing Mei
Mar 3, 2007·Biological & Pharmaceutical Bulletin·Ru-Jiang LiHai-Xu Wang
May 7, 2010·Journal of Biomedicine & Biotechnology·Valérie JaulneauBernard Dumas
Jul 10, 2012·International Journal of Biological Macromolecules·Jun WuShidong Xu
Jan 17, 2013·The Journal of International Medical Research·H T ZhangH L Yang
Feb 14, 2013·International Journal of Biological Macromolecules·Guolin CuiRuiliang Zhu
Jun 12, 2013·PLoS Genetics·Tom V LeeHamed Jafar-Nejad
Nov 10, 2013·PloS One·Rui HuangGuiyuan Ji

❮ Previous
Next ❯

Citations

Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Changming ZhouRuiliang Zhu
Oct 16, 2018·Frontiers in Microbiology·Zhenhong SunKai Wei

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay
ELISA
ion-exchange chromatography
size-exclusion chromatography
Gel filtration

Software Mentioned

ASTRA

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.