Characterization and comparative analysis of a new mouse microglial cell model for studying neuroinflammatory mechanisms during neurotoxic insults

Neurotoxicology
Souvarish SarkarAnumantha G Kanthasamy

Abstract

Microglia are the first responders of the central nervous system, acting as the key modulators of neuroinflammation observed during neurotoxic insults as well as in the pathophysiology of several neurodegenerative disorders including Alzheimer's (AD), Parkinson's (PD), and Huntington's diseases (HD). The number of publications on microglia has increased steadily throughout the past decade because of immense interests in the neuroinflammation that precedes the neurodegenerative process. To study microglial biology and its role in modulating neuroinflammation, immortalized microglial cell lines derived from mice, rats, and humans have been developed. Among these, the BV2 mouse microglial cell line is the most well characterized and widely used cell culture model. However, even unstimulated BV2 cells exhibit an amoeboid, hypertrophied morphology, indicating a highly activated and inflammatory state compared to primary microglia, thus making them less than ideal for studying the low-dose effects of toxicants on microglial activation. Therefore, we performed an in-depth characterization of a recently developed mouse microglial cell (MMC) line, which we compared with primary mouse microglia (PMG) and BV2s to identify which cell line ...Continue Reading

Citations

May 1, 2019·The Journal of Experimental Medicine·Nikhil PanickerArthi Kanthasamy
Feb 23, 2020·Frontiers in Immunology·Souvarish SarkarSrikant Rangaraju
Jul 1, 2020·The Journal of Clinical Investigation·Souvarish SarkarAnumantha G Kanthasamy
Mar 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Supriya RameshaSrikant Rangaraju

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