Characterization and comparative analysis of toxin-antitoxin systems in Acetobacter pasteurianus

Journal of Industrial Microbiology & Biotechnology
Kai XiaXinle Liang

Abstract

Bacterial toxin-antitoxin (TA) systems play important roles in diverse cellular regulatory processes. Here, we characterize three putative type II TA candidates from Acetobacter pasteurianus and investigate the profile of type II TA systems in the genus Acetobacter. Based on the gene structure and activity detection, two-pairs loci were identified as the canonical hicAB and higAB TA systems, respectively, and DB34_01190-DB34_01195 as a putative new one without a canonical TA architecture. Physiologically, the expression of the three pairs conferred E. coli with additional plasmid maintenance and survival when under acetic acid stress. Chromosomal TA systems can be horizontally transferred within an ecological vinegar microbiota by co-option, and there was a tendency for toxin module loss. The antitoxin retention in the genome is suggested to have a broad role in bacterial physiology. Furthermore, A. pasteurianus strains, universally domesticated and used for industrial vinegar fermentation, showed a higher number of type II TA loci compared to the host-associated ones. The amount of TA loci per genome showed little positive relationship to insertion sequences, although its prevalence was species-associated, to the extent of eve...Continue Reading

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Citations

Jun 15, 2020·Applied Microbiology and Biotechnology·Jun XuXinle Liang

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Datasets Mentioned

BETA
CP012111

Methods Mentioned

BETA
PCR

Software Mentioned

ISfinder
mosAT
tBLASTn
ClustalW
Origin
SWISS
MODEL

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