Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Heliyon
Benita QuilodránDavid Silva

Abstract

The thraustochytrid are marine heterotrophic protists that are widely distributed in the marine world. They are characterized by producing and accumulating great amount of lipids in their cells, especially long chain polyunsaturated fatty acids (LC-PUFA), highlighting the docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3) and arachidonic acid (ARA, 20:4, n-6), as well as pigments of interest for human health and animal nutrition, such as carotenoids. Therefore, the objective of this study was to isolate and characterize three natives isolated of thraustochytrids and assess the potential of the by-products of the manufacture of beer (RB) and protein extraction of Lupine flour (RL) as complex carbon sources to produce biomass, lipid and polyunsaturated fatty acids. Three native strains of thraustochytrid (AS5-B2, IQ81 y VAL-B1), isolated from Chilean coastal waters were morphologically and genetically identified as thraustochytrid. For the determination of biomass production cultures were quantified by gravimetry and the fatty acids quantification and identification were carried out by gas chromatography (GC-FID). Our results show that the culture with any sources of complex carbon used, increased signi...Continue Reading

References

Dec 29, 1999·Marine Biotechnology·T E LewisT A McMeekin
Dec 21, 2000·Journal of Microbiological Methods·T LewisT A McMeekin
Jan 22, 2003·Applied Microbiology and Biotechnology·J K Volkman
May 15, 2007·Journal of Agricultural and Food Chemistry·Adam M BurjaColin J Barrow
Jan 19, 2010·Bioresource Technology·Yanna LiangJames W Blackburn
Jan 10, 2012·Progress in Molecular and Subcellular Biology·Lucia Bongiorni
Mar 13, 2014·Current Opinion in Biotechnology·T Catalina Adarme-VegaPeer M Schenk
May 9, 2014·Applied Microbiology and Biotechnology·Purnima SinghGuangyi Wang
Jun 28, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Esteban CaamañoAlex R González
Sep 6, 2017·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Francisca EcheverríaAlfonso Valenzuela
Jul 27, 2018·Journal of Advanced Research·María P Villarroel Hipp, David Silva Rodríguez
Apr 11, 2019·Current Medicinal Chemistry·Rodrigo ValenzuelaLuis Alberto Videla

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
protein extraction

Software Mentioned

GraphPadPrism
ChemStation chromatographic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.