Characterization and Comparison of Protein and Peptide Profiles and their Biological Activities of Improved Common Bean Cultivars (Phaseolus vulgaris L.) from Mexico and Brazil

Plant Foods for Human Nutrition
Luis Mojica, Elvira González de Mejía

Abstract

Common bean (Phaseolus vulgaris L.) is a good source of protein, vitamins, minerals and complex carbohydrates. The objective was to compare protein profile, including anti-nutrient proteins, and potential bioactive peptides of improved common bean cultivars grown in Mexico and Brazil. Bean protein isolates (BPI) were prepared from 15 common bean cultivars and hydrolyzed using pepsin/pancreatin. Thirteen proteins were identified by SDS-PAGE and protein in-gel tryptic-digestion-LC/MS. Protein profile was similar among common bean cultivars with high concentrations of defense-related proteins. Major identified proteins were phaseolin, lectin, protease and α-amylase inhibitors. Lectin (159.2 to 357.9 mg lectin/g BPI), Kunitz trypsin inhibitor (inh) (4.3 to 75.5 mg trypsin inh/g BPI), Bowman-Birk inhibitor (5.4 to 14.3 μg trypsin-chymotrypsin inh/g BPI) and α-amylase inhibitor activity (2.5 to 14.9% inhibition relative to acarbose/mg BPI) were higher in Mexican beans compared to Brazilian beans. Abundant peptides were identified by HPLC-MS/MS with molecular masses ranging from 300 to 1500 Da and significant sequences were SGAM, DSSG, LLAH, YVAT, EPTE and KPKL. Potential bioactivities of sequenced peptides were angiotensin converting...Continue Reading

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Jun 22, 2014·Plant Foods for Human Nutrition·Ana Angelica Feregrino-PerezGuadalupe Loarca-Piña

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Citations

Dec 3, 2015·Plant Foods for Human Nutrition·Ana Clara SabbioneAdriana A Scilingo
Sep 4, 2016·Plant Foods for Human Nutrition·Abraham Wall-MedranoRamón Robles-Zepeda
Sep 30, 2016·Plant Foods for Human Nutrition·Raheleh JahanbaniAli Akbar Moosavi-Movahedi
Feb 2, 2018·Critical Reviews in Food Science and Nutrition·Domancar Orona-TamayoOctavio Paredes-López
Jun 2, 2020·Critical Reviews in Food Science and Nutrition·Bianca de Fátima GarciaThaís de Souza Rocha
Mar 23, 2017·International Journal of Food Science·Aline P OliveiraJuliana Naozuka
Feb 6, 2020·Frontiers in Genetics·Manvi SharmaTrygve O Tollefsbol
Aug 5, 2017·Journal of Agricultural and Food Chemistry·Gabriela Barbosa RossiAna Carolina Maisonnave Arisi

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