Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments of Mongolian horses.

MicrobiologyOpen
Shaofeng SuManglai Dugarjaviin

Abstract

The intestinal microbiota plays an important role in the health and metabolism of the host. Next-generation sequencing technology has enabled the characterization of the gut microbiota of several animal species. We analyzed the intestinal microbiota in six different parts of the gastrointestinal tracts (GITs) of five Mongolian horses by sequencing the 16S rRNA gene V3-V4 hypervariable region. All horses were kept in the natural habitat of the Inner Mongolia grassland. Significant differences were observed among the microbiota compositions of the distinct GIT regions. In addition, while the microbial community structures of the small and large intestine were significantly different, those of the cecum and colon were similar. In the foregut, Firmicutes (65%) and Proteobacteria (23%) were the most abundant, while Firmicutes (45%) and Bacteroidetes (42%) were the most common in the hindgut. At the level of family, Ruminococcaceae (p = .203), Lachnospiraceae (p = .157), Rikenellaceae (p = .122), and Prevotellaceae (p = .068) were predominant in the hindgut, while the relative abundance of the Akkermansia genus (5.7%, p = .039) was higher in the ventral colon. In terms of the putative functions, the ratio of microbial abundance in th...Continue Reading

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Citations

Feb 24, 2021·Scientific Reports·Kailee J ReedStephen J Coleman
Apr 7, 2021·The Veterinary Clinics of North America. Equine Practice·Patricia Harris, Megan Shepherd
Oct 26, 2021·Frontiers in Veterinary Science·Chongyu ZhangYunkyoung Lee

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Methods Mentioned

BETA
electrophoresing
PCR
phosphotransferase
metabolomic profiling
amplicon sequencing

Software Mentioned

PICRUSt
Uparse
Mothur
QIIME
PCoA
SPSS

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