PMID: 9185573Jul 1, 1997Paper

Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein

Nucleic Acids Research
L E BirdD B Wigley

Abstract

Limited proteolysis of bacteriophage T7 primase/helicase with endoproteinase Glu-C produces several proteolytic fragments. One of these fragments, which is derived from the C-terminal region of the protein, was prepared and shown to retain helicase activity. This result supports a model in which the gene 4 proteins consist of functionally separable domains. Crystals of this C-terminal fragment of the protein have been obtained that are suitable for X-ray diffraction studies.

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Citations

Mar 31, 1998·Current Opinion in Structural Biology·L E BirdD B Wigley
Mar 5, 2009·Biochemistry·Lauren P BlairKevin D Raney
Nov 28, 2007·Nucleic Acids Research·Géraldine FargeMaria Falkenberg
Mar 31, 2010·Nucleic Acids Research·Seung-Joo LeeCharles C Richardson
May 22, 2002·Protein Science : a Publication of the Protein Society·Yanshun Liu, David Eisenberg
Jun 8, 2001·Annual Review of Biochemistry·D N Frick, C C Richardson
Sep 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Seung-Joo Lee, Charles C Richardson
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D N FrickC C Richardson
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KusakabeC C Richardson
Dec 1, 2015·Critical Reviews in Biochemistry and Molecular Biology·Laurie S Kaguni, Marcos T Oliveira
Mar 30, 2010·Methods : a Companion to Methods in Enzymology·Magdalena M Makowska-GrzyskaLaurie S Kaguni
Feb 28, 2007·Journal of Molecular Biology·Tawn D ZiebarthLaurie S Kaguni
Feb 24, 2006·Journal of Molecular Biology·Rafael Núñez-RamírezLuis Enrique Donate
Aug 31, 2000·Annual Review of Biochemistry·S S Patel, K M Picha
Nov 26, 2003·Molecular Cell·Eric A TothTom Ellenberger
Mar 30, 1999·Biochimica Et Biophysica Acta·L E Bird, D B Wigley
Nov 2, 1999·Journal of Molecular Biology·H GeorgeS C West
Dec 10, 1999·The Journal of Biological Chemistry·D N Frick, C C Richardson
Apr 5, 2000·The Journal of Biological Chemistry·A C DéclaisM Duguet
Oct 24, 2001·The Journal of Biological Chemistry·S J Lee, C C Richardson
May 11, 2004·The Journal of Biological Chemistry·Masato KatoTom Ellenberger
Mar 27, 2004·The Journal of Biological Chemistry·Zheng-Guo He, Charles C Richardson
Mar 10, 2010·The Journal of Biological Chemistry·Tawn D ZiebarthLaurie S Kaguni
Mar 30, 2001·The Journal of Biological Chemistry·M KatoT Ellenberger

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