PMID: 8970814Sep 15, 1996Paper

Characterization and development of an inner ear type I fibrocyte cell culture

Hearing Research
M A GrattonDebra J Hazen-Martin

Abstract

A method has been developed that allows successful maintenance of secondary cell cultures derived from explants of the cochlear lateral wall of young adult gerbils. The secondary cultures were characterized morphologically with light and transmission electron microscopy and immunocytochemically with protein markers specific to various lateral wall cell types. Structural studies revealed fusiform-shaped cells with a paucity of cytoplasm surrounding the nucleus and slender processes. The cells showed little evidence of intercellular contact even when confluent. The cultures were immunopositive for vimentin, carbonic anhydrase isozyme II, creatine kinase isozyme BB and smooth endoplasmic reticulum Ca-ATPase, but lacked reactivity for cytokeratins and Na,K-ATPase. The results indicate that the cultures are comprised of type I fibrocytes from the spiral ligament. These findings are the first to demonstrate that inner ear spiral ligament cells can be isolated and maintained in secondary culture while retaining many of their in vivo characteristics. Based upon their location and content of ion transport enzymes, type I fibrocytes are thought to be involved in the recycling of potassium from perilymph into the stria vascularis. The est...Continue Reading

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Citations

Mar 6, 2007·Journal of the Association for Research in Otolaryngology : JARO·Chunyan QuBradley A Schulte
Apr 6, 2012·Journal of the Association for Research in Otolaryngology : JARO·John J KellyDaniel J Jagger
Nov 14, 2000·International Journal of Pediatric Otorhinolaryngology·I IchimiyaG Mogi
Feb 17, 2000·Hearing Research·T SukoG Mogi
Nov 2, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shanthini MahendrasingamDavid N Furness
Apr 27, 2012·Journal of Neuroscience Research·Guang-Wei SunTatsuo Matsunaga
May 25, 2006·Acta Oto-laryngologica·Sung-Kyun MoonDavid J Lim
Jun 15, 2004·Brain Research. Molecular Brain Research·Fenghe LiangZhijun Shen
Jan 12, 2007·The Annals of Otology, Rhinology, and Laryngology·Christopher YianDavid J Lim
Mar 13, 2003·Pflügers Archiv : European journal of physiology·F LiangZ Shen
Aug 6, 2019·Frontiers in Molecular Neuroscience·Toshiya MinakataShingo Murakami

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