PMID: 8589415Jul 1, 1995Paper

Characterization and disruption of a gene in the maize pathogen Cochliobolus carbonum encoding a cellulase lacking a cellulose binding domain and hinge region

Molecular Plant-microbe Interactions : MPMI
P SposatoJ D Walton

Abstract

A gene, CEL1, in the maize pathogen Cochliobolus carbonum was identified using the cbh1-3 gene of Phanerochaete chrysosporium as a heterologous probe. The predicted product of CEL1, Cel1, is 62% identical and 71% similar to the product of cbh1-3 and 54 to 62% identical to five cellobiohydrolases from other filamentous fungi. The location of the polyadenylation site 221 bp downstream of the stop codon and the location of a single intron of 55 bp were identified by comparison of the sequences of genomic and cDNA copies of CEL1. The transcriptional start site was determined by rapid amplification of cDNA ends (RACE) to be 39 bp upstream of the putative translational start site. CEL1 mRNA abundance is high when C. carbonum is grown on cellulose or maize cell walls but is undetectable when grown on 2% sucrose or cellulose plus sucrose. Cel1 has a predicted signal peptide of 18 amino acids and therefore a mature size of 46.4 kDa. Like the product of cbh1-1 of P. chrysosporium, but unlike most other endoglucanases and cellobiohydrolases (including the predicted product of cbh1-3), Cel1 does not have a putative cellulose binding domain or associated hinge region. The codon bias of CEL1 is stronger than the bias of cbh1-1 and comparable...Continue Reading

Citations

Nov 12, 2005·Current Genetics·Vincent PhalipJean-Marc Jeltsch
May 19, 2006·Biochemical and Biophysical Research Communications·Didier HatschJean-Marc Jeltsch
May 20, 2000·Biotechnology & Genetic Engineering Reviews·R A PradeA J Mort
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Jorrit-Jan KrijgerStefan G R Wirsel
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·A ten HaveJ A van Kan
Jan 1, 1996·Annual Review of Phytopathology·K MendgenH Deising
Jan 1, 1997·Annual Review of Phytopathology·R A Dean
Jul 27, 2001·Bioscience, Biotechnology, and Biochemistry·J H AhnJ D Walton
Apr 9, 2013·Phytochemistry·Olivier HabryloVincent Phalip
Jul 4, 2008·Fungal Genetics and Biology : FG & B·Mihwa YiYong-Hwan Lee
Jul 18, 2001·Fungal Genetics and Biology : FG & B·A ten HaveJ A van Kan
Feb 6, 2008·Fungal Genetics and Biology : FG & B·Raphaël CarapitoVincent Phalip
Feb 1, 1997·Fungal Genetics and Biology : FG & B·J E Hamer, D W Holden
Jan 15, 2013·FEMS Microbiology Letters·Raphaël CarapitoVincent Phalip
May 19, 2009·Molecular Plant-microbe Interactions : MPMI·E RemyT Rouxel
May 9, 1998·Applied and Environmental Microbiology·M A VallimD Cullen
Apr 18, 1998·Applied and Environmental Microbiology·J S Scott-CraigJ D Walton
Nov 1, 1996·Applied and Environmental Microbiology·P C Apel-Birkhold, J D Walton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.