Characterization and evaluation of an arbitrary primed Polymerase Chain Reaction (PCR) product for the specific detection of Brucella species

Saudi Journal of Biological Sciences
Jafar A QasemVinod Kumar

Abstract

Laboratory detection of Brucella is based largely on bacterial isolation and phenotypic characterization. These methods are lengthy and labor-intensive and have been associated with a heightened risk of laboratory-acquired infection. Antibody based indirect detection methods also suffer from limitations in proper diagnosis of the organism. To overcome these problems, nucleic acid amplification has been explored for rapid detection and confirmation of the presence of Brucella spp. PCR-based diagnostics is useful for screening large populations of livestock to identify infected individuals and confirms the presence of the pathogen. Random Amplification of Polymorphic DNA (RAPD) was performed and identified a 1.3 kb PCR fragment specifically amplifiable from DNA isolated from Brucella. A BLAST search revealed no significant homology with the reported sequences from species other than the members of Brucella. The isolated fragment seems to be a part of d-alanine-d-alanine ligase gene in Brucella sp. Translational BLAST revealed certain degree of homology of this sequence with orthologs of this gene reported from other microbial species at the deduced amino acid level. The sequence information was used to develop PCR based assays to...Continue Reading

References

Oct 1, 1992·Applied Microbiology and Biotechnology·B J BassamP M Gresshoff
Jan 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A FeketeS M Halling
Jan 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J ArizaF Gudiol
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 1, 1990·The Journal of Applied Bacteriology·A FeketeM R Sanborn
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·A M MousaT N Sugathan
Feb 1, 1995·Veterinary Microbiology·B J WardsG W de Lisle
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 15, 2001·Current Opinion in Microbiology·M L BoschiroliD O'Callaghan
Nov 5, 2002·Veterinary Microbiology·Betsy J Bricker
Jun 3, 2005·The New England Journal of Medicine·Georgios PappasEpameinondas Tsianos
Dec 20, 2005·Journal of Korean Medical Science·Mi-Yeoun ParkHeung-Bum Lee
Sep 29, 2007·Indian Journal of Medical Microbiology·B G ManturR S Shinde
Mar 26, 2011·BMC Microbiology·Riccardo De SantisFlorigio Lista

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