Characterization and expression of digestive neutral lipases during ontogeny of Atlantic cod (Gadus morhua)

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Øystein SaeleKristin Hamre

Abstract

The major neutral lipase excreted by the pancreas in fish, is bile activated lipase (BAL). Here we present evidence that cod have a functional BAL and a non-functional pancreatic lipase related protein (PLRP). The Atlantic cod genome does not seem to contain colipase which is essential for pancreatic lipase activity. During the larval stages, the gene expression of BAL was low until the point when pyloric caeca started to differentiate and develop (approximately 20mm standard length (SL)). Then the expression increased until approximately 50mm SL. The PLRP gene was expressed but showed very little regulation. The activity of neutral lipase did not increase in parallel to gene expression. The mismatch between activity and gene expression measurements may be partly explained by the unspecific analytical method, when analysing lipase activity in larva whole body. There is neutral lipase activity in numerous tissues in the fish larvae and the lipase activity in the gut, relatively to activity in the whole body, decreased with age. Furthermore, neutral lipase activity in rotifers was ten times higher than in whole cod larvae with full guts. Activity originating from the live prey may therefore explain the high whole body lipase acti...Continue Reading

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Citations

Jul 21, 2012·The British Journal of Nutrition·Øystein SæleKristin Hamre
Dec 25, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Truls Wergeland HansenØystein Sæle
Mar 6, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Manee SrichanunTrond M Kortner
Jul 25, 2014·Fish Physiology and Biochemistry·Koji MurashitaTakeshi Yamamoto
Sep 30, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·José Antonio Mata-SotresGonzalo Martínez-Rodríguez
Aug 28, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Keshuai LiYngvar Olsen
Jun 21, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Trond M KortnerAugustine Arukwe
May 31, 2014·Free Radical Biology & Medicine·Kristin HamrePål A Olsvik
May 10, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Stefan de GelderPeter H M Klaren
Nov 2, 2018·Fish Physiology and Biochemistry·Leire ArantzamendiMarisol Izquierdo
Apr 6, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Neda GilannejadGonzalo Martínez-Rodríguez
Feb 20, 2020·Proceedings. Biological Sciences·Joseph HerasDonovan P German
May 24, 2020·Animals : an Open Access Journal From MDPI·Neda GilannejadGonzalo Martínez-Rodríguez
Jun 21, 2019·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Ishfaq Nazir MirK K Jain

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