Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)

Scientific Reports
Liying YuMinsheng You

Abstract

Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxifica...Continue Reading

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Citations

Feb 24, 2016·Frontiers in Physiology·Vijaya Sudhakara Rao KolaMaganti S Madhav
Aug 1, 2015·Genome Biology and Evolution·Martijn F L DerksHendrik-Jan Megens
Jun 27, 2018·Journal of Economic Entomology·Reihaneh BaratiSeyed Abolghasem Mohammadi
Oct 21, 2018·Planta·Linhong TengNaihao Ye
Jul 8, 2017·BMC Evolutionary Biology·Qian ZhaoMinsheng You
Jun 26, 2020·Communications Biology·Khaled Michel HazzouriKhaled M A Amiri
Feb 20, 2021·PeerJ·Linrong WanUNKNOWN members of the Genefang Research Team: Zi-yan Huang, Cong-fan Bu, Jie Zeng, Zhao-nan Hao, Yan-peng Chen, and Meng-jia Liu
Jul 3, 2021·Insects·Frédérique HilliouGaëlle Le Goff

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Methods Mentioned

BETA
environmental stress
RNA-seq
PCR

Software Mentioned

BLAST
SPSS
Primer premier
Cluster
hmmsearch
hmmscan
HMMER3
MEGA5
DB
DBM

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