Characterization and expression of two cDNAs encoding carbonic anhydrase in Arabidopsis thaliana

Plant Physiology
J P Fett, John R Coleman

Abstract

Two distinct cDNA clones encoding carbonic anhydrase (CA) were isolated from an Arabidopsis thaliana lambda YES library. One of these clones, CA1, encodes a 36.1-kD polypeptide and is essentially the same as a previously reported Arabidopsis CA cDNA (C.A. Raines, P.R. Horsnell, C. Holder, J.C. Lloyd [1992] Plant Mol Biol 20: 1143-1148). Comparison of the derived amino acid sequence from this clone with other plant CAs suggests the presence of a chloroplastic transit peptide, which, when cleaved, would render a mature protein of 24.3 kD. The other identified clone, CA2, encodes a 28.3-kD polypeptide, which in addition to other residue changes, is 78 amino acids shorter at the N terminus than the primary product of CA1. The two cDNAs exhibit 76.9% sequence similarity at the DNA level and 84.6% identity between the predicted amino acid sequences. A polyclonal antibody generated against pea CA (N. Majeau, J.R. Coleman [1991] Plant Physiol 100: 1077-1078) hybridized to two protein bands (25 and 28 kD) from a total leaf extract and to only one band (25 kD) from a chloroplastic protein extract. The data suggest that the CA2 protein is an extrachloroplastic form of CA, presumably localized in the cytoplasm. Southern analysis indicated ...Continue Reading

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Citations

Feb 16, 2011·Journal of Experimental Botany·Sylvain AubryJulian M Hibberd
Mar 17, 2011·Journal of Experimental Botany·Martha Ludwig
Nov 18, 2000·Plant Physiology·S GálvezM Crespi
Aug 2, 2005·The Plant Cell·Naomi NakayamaVivian F Irish
Jan 23, 2014·Parasites & Vectors·Reza Zolfaghari EmamehSeppo Parkkila
Jan 5, 2014·Planta·Werner E G MüllerXiaohong Wang
Feb 3, 2000·Molecular Plant-microbe Interactions : MPMI·N KavroulakisP Katinakis
Aug 15, 2009·Biochimica Et Biophysica Acta·Roger S Rowlett
Jun 3, 2011·Plant, Cell & Environment·Martha Ludwig
Sep 6, 2014·PloS One·Elisa VanzoJörg-Peter Schnitzler
Feb 27, 2013·Journal of Proteome Research·Stephen ChivasaAntoni R Slabas
Sep 21, 2016·Molecular Plant·Robert J DiMarioJames V Moroney
Feb 6, 2017·Plant Physiology and Biochemistry : PPB·Jolanta Floryszak-Wieczorek, Magdalena Arasimowicz-Jelonek
Jun 11, 2004·The Journal of Biological Chemistry·Sébastien MongrandJean-Jacques Bessoule
Nov 5, 2015·Biochemistry. Biokhimii︠a︡·N N RudenkoB N Ivanov
Apr 19, 2019·Metabolites·Lyudmila IgnatovaBoris Ivanov
Oct 30, 2020·Protoplasma·Natalia N RudenkoMaria M Borisova-Mubarakshina

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