PMID: 9428656Jan 15, 1998Paper

Characterization and functional properties of the alpha-amylase inhibitor (alpha-AI) from kidney bean (Phaseolus vulgaris) seeds

Biochimica Et Biophysica Acta
V Le Berre-AntonP Rougé

Abstract

Alpha-amylase inhibitor (alpha-AI) from kidney bean (Phaseolus vulgaris L. cv Tendergreen) seeds has been purified to homogeneity by heat treatment in acidic medium, ammonium sulphate fractionation, chromatofocusing and gel filtration. Two isoforms, alpha-AI1 and alpha-AI1', of 43 kDa have been isolated which differ from each other by their isoelectric points and neutral sugar contents. The major isoform alpha-AI1 inhibited human and porcine pancreatic alpha-amylases (PPA) but was devoid of activity on alpha-amylases of bacterial or fungal origins. As shown on the Lineweaver-Burk plots, the nature of the inhibition is explained by a mixed non-competitive inhibition mechanism. Alpha-AI1 formed a 1:2 stoichiometric complex with PPA which showed an optimum pH of 4.5 at 30 degrees C. Owing to the low optimum pH found for alpha-AI activity, inhibitor-containing diets such as beans or transgenic plants expressing alpha-AI should be devoid of any harmful effect on human health.

References

Oct 1, 1978·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K H Pick, G Wöber
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J Moreno, M J Chrispeels
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Dec 2, 1982·The New England Journal of Medicine·I H Rosenberg
Feb 1, 1984·The American Journal of Clinical Nutrition·I E LienerJ C Tarcza
Mar 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M IshimotoK Kitamura

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Citations

Feb 12, 2004·Biochimica Et Biophysica Acta·Daniela BellincampiDonatella Vairo
Mar 20, 1999·Biochimica Et Biophysica Acta·S DarnisJ C Chaix
Dec 19, 2003·Protein Expression and Purification·H X Wang, T B Ng
Mar 12, 2008·The British Journal of Nutrition·Wokadala Cuthbert ObiroBo Jiang
Feb 22, 2002·European Journal of Biochemistry·Octávio L FrancoMaria F Grossi-De-Sá
Aug 10, 2000·European Journal of Biochemistry·E J Van DammeW J Peumans
Jul 23, 2005·International Journal of Toxicology·Kuzhuvelil B HarikumarRamadasan Kuttan
Jan 29, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Shiva Khalil-MoghaddamNasim Hayati-Roodbari
Nov 11, 2010·Journal of Insect Science·Mohammad MehrabadiFatemeh Saadati
May 8, 2009·Bioscience, Biotechnology, and Biochemistry·Xiaoyan HaoHao Ma
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·R L MortonT J Higgins
Mar 22, 2014·Journal of Medicinal Food·Neire Moura de GouveiaFoued Salmen Espindola
Sep 21, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Vincent S M Ho, T B Ng
May 13, 2015·Bioorganic Chemistry·Irshad Ali VeesarShahabuddin Memon
Aug 15, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Patrick H K Ngai, T B Ng
Jul 7, 2020·Journal of Food Science and Technology·Rakrudee SarnthimaAnupong Joompang
Aug 18, 2020·Biochimica Et Biophysica Acta. General Subjects·Ashwini S RaneAshok P Giri
Jan 27, 2010·Journal of Agricultural and Food Chemistry·Anussorn WisessingKiattawee Choowongkomon
Sep 4, 2007·Plant Physiology and Biochemistry : PPB·A M Guzman-PartidaL Vazquez-Moreno

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