Characterization and functional role of the QH site of bo-type ubiquinol oxidase from Escherichia coli

Biochemistry
M Sato-WatanabeY Anraku

Abstract

Cytochrome bo is a four-subunit terminal ubiquinol oxidase in the aerobic respiratory chain of Escherichia coli that vectorially translocates protons not only via directed protolytic reactions but also via proton pumping. Previously, we postulated that a bound quinone in the high-affinity quinone binding site (QH) mediates electron transfer from the low-affinity quinol oxidation site (QL) in subunit II to low-spin heme b in subunit I as an electron gate and a transient electron reservoir [Sato-Watanabe, M., Mogi, T., Ogura, T., Kitagawa, T., Miyoshi, H., Iwamura, H., and Anraku, Y. (1994b) J. Biol. Chem. 269, 28908-28912]. In the present study, we carried out screening of ubiquinone analogues using a bound ubiquinone-free enzyme (DeltaUbiA1) that has been isolated from a ubiquinone biosynthesis mutant, and identified PC24 (2-chloro-4, 6-dinitrophenol), PC32 (2,6-dibromo-4-cyanophenol), and PC52 (2-isopropyl-5-methyl-4,6-dinitrophenol) as potent QH site inhibitors. PC15 (2,6-dichloro-4-nitrophenol) and PC16 (2, 6-dichloro-4-dicyanovinylphenol), potent QL site inhibitors, did not exhibit such a selective inhibition of the QH site. Binding studies using the air-oxidized DeltaUbiA enzyme showed that PC32 and PC52 have 4- to 7-fold ...Continue Reading

References

Aug 1, 1991·Biochemical Society Transactions·M SarasteJ van der Oost
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Citations

Jul 9, 2009·Bioscience, Biotechnology, and Biochemistry·Kazuo KobayashiTatsushi Mogi
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·T K DasD L Rousseau
Feb 5, 2013·Biochimica Et Biophysica Acta·Stéphane GrimaldiAxel Magalon
Apr 27, 2010·Biochimica Et Biophysica Acta·Lai Lai YapRobert B Gennis
May 26, 2017·Journal of the American Chemical Society·Sylvia K ChoiRobert B Gennis
May 5, 2006·Journal of Biochemistry·Yushi MatsumotoTatsushi Mogi
Mar 7, 2012·The Journal of Biological Chemistry·Duncan G G McMillanLars J C Jeuken
Feb 12, 2019·Chemical Reviews·Kazuo Kobayashi

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