Characterization and functions of vascular adventitial fibroblast subpopulations

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Sheng Jun AnJun Qiu Fu

Abstract

Adventitial fibroblasts have been shown to play an important role in vascular remodeling and contribute to neointimal formation in vascular diseases. However, little is known about adventitial fibroblast subpopulations. This study explored the process of isolating rat thoracic aorta adventitial fibroblast subpopulations and characterized their properties following stimulation with angiotensin II (ANG II), a critical factor involved in cardiovascular diseases such as hypertension. Adventitial fibroblasts were isolated and cultured from rat aorta. Fibroblast subpopulations were individually expanded using cloning ring techniques. Cells were treated with ANG II (10 nM, 100 nM and 1 μM) for 0.5, 1, 1.5, 3, 6, 12, or 24 h, and ANG II-induced proliferation and migration were measured by MTT assay and Transwell. Cells were treated with ANG II (100 nM) in the presence or absence of ANG II receptor antagonists (100 μM), losartan (for AT1) and PD-123319 (for AT2). PreproET-1 mRNA and ET-1 were determined by RT-PCR and ELISA, respectively. Collagen type I was detected by western blotting. Two major fibroblast subpopulations were found in the adventitia, epithelioid-like cells and spindle-like cells; Although ANG II promotes the growth of ...Continue Reading

Citations

Jul 15, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Jill T Kuwabara, Michelle D Tallquist
Nov 11, 2015·Hypertension·Daniel N Meijles, Patrick J Pagano
May 26, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Ariel WangDaniela Valdez-Jasso
Sep 1, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jie LuoDi Zheng
Nov 17, 2020·Journal of the American Heart Association·Laura E BruijnJan H N Lindeman
Dec 24, 2019·The American Journal of Pathology·Maria G Tinajero, Avrum I Gotlieb
Jun 9, 2021·Biofabrication·Faraz FazalNorbert Radacsi
Jul 16, 2021·Frontiers in Cell and Developmental Biology·Hui XuYou-Shuo Liu

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