Abstract
Follicular B cell lymphomas of SJL mice [reticulum cell sarcoma (RCS)] are dependent on syngeneic Ly-1+,2- T cells for their growth. These T cells produce a number of lymphokines in response to stimulation with gamma-irradiated RCS cells including interleukin (IL) 2, interferon-gamma (IFN-gamma), IL4 and IL5, some of which may be required for growth of the tumor. Previous studies have shown that an RCS cell line, cRCS-X, can be maintained in vitro indefinitely, if the cultures are supplemented with gamma-irradiated lymph node (LN) cells or with a preparation of human B cell growth factor (BCGF). In the present studies, the growth requirements of this cell line were analyzed in more detail in short-term assays of both [3H]thymidine incorporation and colony formation in agarose. Recombinant murine IL5 cause dose-dependent proliferation of cRCS-X cells similar to that induced with BCGF. The level of colony formation by cRCS-X cells induced by optimal concentrations of BCGF was not increased further by the addition of IL5, suggesting that the two factors act via a common mechanism. IL1 and IFN-gamma each enhanced cRCS-X proliferation induced by BCGF or IL5 in both assays. The effects of IL1 plus BCGF, IFN-gamma plus BCGF, and IL1 p...Continue Reading
References
Apr 1, 1976·Cellular Immunology·S P LermanG J Thorbecke
Mar 1, 1979·Cellular Immunology·S P LermanG J Thorbecke
May 1, 1976·The Journal of Experimental Medicine·P W KincadeM A Moore
May 23, 1985·Nature·J Ohara, W E Paul
Feb 1, 1988·Immunological Reviews·R L CoffmanM W Bond
Jul 1, 1988·The Journal of Experimental Medicine·S KarrayP Galanaud
Jan 1, 1988·European Journal of Immunology·E Petit-KoskasJ P Kolb
Jul 1, 1986·International Reviews of Immunology·N M PonzioG J Thorbecke
Sep 1, 1974·Cellular Immunology·E B JacobsonG J Thorbecke
Dec 15, 1974·International Journal of Cancer. Journal International Du Cancer·S P LermanG J Thorbecke
Jan 1, 1983·Immunogenetics·P H BrownG J Thorbecke
Sep 1, 1983·The Journal of Experimental Medicine·S L SwainR W Dutton
Jul 22, 1982·Nature·G KraalE C Butcher
Mar 1, 1982·The Journal of Experimental Medicine·M HowardW E Paul
Jan 1, 1982·Immunogenetics·C MarkJ Klein
Feb 1, 1980·The Journal of Experimental Medicine·I R KatzG J Thorbecke
May 1, 1960·Cancer·A L SOPCHAK, A M SUTHERLAND
Citations
Jan 1, 1997·Muscle & Nerve·A H WellerN L Rosenberg
Jan 1, 1996·Springer Seminars in Immunopathology·N M PonzioG J Thorbecke
Oct 1, 1995·Molecular Immunology·H LuoS R King
Aug 11, 2000·Leukemia Research·G S Erianne1N M Ponzio
May 15, 2002·Leukemia Research·Janine WajchmanNicholas M Ponzio
Oct 2, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·R Dolcetti, M Boiocchi
Jun 14, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hai LiVincent K Tsiagbe
Feb 1, 1993·Cell and Tissue Research·A R CafféT van Veen
Jan 8, 2017·Molecular & Cellular Proteomics : MCP·Harvey E JohnstonMark S Cragg
Apr 1, 1992·Immunological Reviews·V K TsiagbeG J Thorbecke
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N SenG J Thorbecke
Dec 2, 2000·Seminars in Cancer Biology·N M Ponzio, G J Thorbecke
Mar 1, 1995·Human Pathology·R DolcettiM Boiocchi