Characterization and growth factor requirements of SJL lymphomas. II. Interleukin 5 dependence of the in vitro cell line, cRCS-X, and influence of other cytokines

European Journal of Immunology
J L Lasky, G J Thorbecke

Abstract

Follicular B cell lymphomas of SJL mice [reticulum cell sarcoma (RCS)] are dependent on syngeneic Ly-1+,2- T cells for their growth. These T cells produce a number of lymphokines in response to stimulation with gamma-irradiated RCS cells including interleukin (IL) 2, interferon-gamma (IFN-gamma), IL4 and IL5, some of which may be required for growth of the tumor. Previous studies have shown that an RCS cell line, cRCS-X, can be maintained in vitro indefinitely, if the cultures are supplemented with gamma-irradiated lymph node (LN) cells or with a preparation of human B cell growth factor (BCGF). In the present studies, the growth requirements of this cell line were analyzed in more detail in short-term assays of both [3H]thymidine incorporation and colony formation in agarose. Recombinant murine IL5 cause dose-dependent proliferation of cRCS-X cells similar to that induced with BCGF. The level of colony formation by cRCS-X cells induced by optimal concentrations of BCGF was not increased further by the addition of IL5, suggesting that the two factors act via a common mechanism. IL1 and IFN-gamma each enhanced cRCS-X proliferation induced by BCGF or IL5 in both assays. The effects of IL1 plus BCGF, IFN-gamma plus BCGF, and IL1 p...Continue Reading

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Citations

Jan 1, 1997·Muscle & Nerve·A H WellerN L Rosenberg
Jan 1, 1996·Springer Seminars in Immunopathology·N M PonzioG J Thorbecke
May 15, 2002·Leukemia Research·Janine WajchmanNicholas M Ponzio
Oct 2, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·R Dolcetti, M Boiocchi
Jun 14, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hai LiVincent K Tsiagbe
Jan 8, 2017·Molecular & Cellular Proteomics : MCP·Harvey E JohnstonMark S Cragg
Apr 1, 1992·Immunological Reviews·V K TsiagbeG J Thorbecke
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N SenG J Thorbecke

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