Characterization and haplotype analysis of 10 novel Y-STR loci in Chinese Han population

Forensic Science International : Synergy
H L DaiY P Hou

Abstract

In this study, we analyzed allelic sequences of 10 novel Y-specific STR loci, DYS454, DYS510, DYS513, DYS520, DYS542, DYS544, DYS552, DYS561, DYS587 and DYS593, surveyed the distribution of haplotypes in a Chinese Han population. Extracted DNA was amplified with PCR, followed by a horizontal non-denaturing polyacrylamide gel electrophoresis with discontinuous buffer system. Purified alleles were sequenced on DNA sequencer (ABI Model 377) to verify the number of motif repeats. The number of alleles observed at each locus ranged from 3 to 8, yielding 102 haplotypes in 103 unrelated males samples. The allele diversity values for each locus ranged from 0.2099 (DYS544) to 0.7523 (DYS552). The haplotype diversity using all these loci was 0.9998. Our study revealed that they were valuable Y-specific markers for forensic applications.

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Citations

Jul 21, 2007·International Journal of Legal Medicine·Mei Sen ShiBo Hu
May 26, 2005·Forensic Science International : Synergy·L GusmãoUNKNOWN DNA Commission of the International Society of Forensic Genetics
Jun 9, 2005·Legal Medicine·Reiko Iida, Koichiro Kishi
Jun 16, 2011·Croatian Medical Journal·Seema P NairChippy Jagannath
Oct 26, 2012·Genetics and Molecular Research : GMR·G Q ZhangD W Guo
Jan 21, 2010·Journal of Genetics·Sun Wha ParkKi Wha Chung
Sep 26, 2006·International Journal of Legal Medicine·L GusmãoUNKNOWN International Society of Forensic Genetics
Feb 5, 2010·Forensic Science International. Genetics·María Eugenia D'AmatoSean Davison
Jan 18, 2006·Forensic Science International : Synergy·John M ButlerMargaret C Kline
Jan 4, 2007·Journal of Forensic Sciences·Erin K HansonJack Ballantyne
Feb 9, 2012·Molecular Biology Reports·Seema Nair ParvathyChippy Jagannath

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