Characterization and heterologous expression of the neoabyssomicin/abyssomicin biosynthetic gene cluster from Streptomyces koyangensis SCSIO 5802

Microbial Cell Factories
Jiajia TuQinglian Li

Abstract

The deep-sea-derived microbe Streptomyces koyangensis SCSIO 5802 produces neoabyssomicins A-B (1-2) and abyssomicins 2 (3) and 4 (4). Neoabyssomicin A (1) augments human immunodeficiency virus-1 (HIV-1) replication whereas abyssomicin 2 (3) selectively reactivates latent HIV and is also active against Gram-positive pathogens including methicillin-resistant Staphylococcus aureus (MRSA). Structurally, neoabyssomicins A-B constitute a new subtype within the abyssomicin family and feature unique structural traits characteristic of extremely interesting biosynthetic transformations. In this work, the biosynthetic gene cluster (BGC) for the neoabyssomicins and abyssomicins, composed of 28 opening reading frames, was identified in S. koyangensis SCSIO 5802, and its role in neoabyssomicin/abyssomicin biosynthesis was confirmed via gene inactivation and heterologous expression experiments. Bioinformatics and genomics analyses enabled us to propose a biosynthetic pathway for neoabyssomicin/abyssomicin biosynthesis. Similarly, a protective export system by which both types of compounds are secreted from the S. koyangensis producer was identified, as was a four-component ABC transporter-based import system central to neoabyssomicin/abyssom...Continue Reading

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Citations

Jan 9, 2019·Natural Product Reports·Liujie HuoRolf Müller
Apr 24, 2019·Marine Drugs·Alexander A Braddock, Emmanuel A Theodorakis
Jun 27, 2019·Applied Microbiology and Biotechnology·Xuefeng WangWeishan Wang
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Apr 10, 2019·Microbiology·Emmanuele Severi, Gavin H Thomas
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Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Zhenquan LinZihe Liu
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Aug 3, 2018·Journal of Natural Products·Hongbo HuangJianhua Ju
Jul 22, 2020·The Journal of Organic Chemistry·Somayah S ElsayedGilles P van Wezel

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Datasets Mentioned

BETA
MG243704

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

antiSMASH
FramePlot

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