Characterization and identification of a C-terminal amidated mechano growth factor (MGF) analogue in black market products

Rapid Communications in Mass Spectrometry : RCM
Simone EspositoPeter Van Eenoo

Abstract

Mechano growth factor (MGF) is a splice variant of insulin-like growth factor that possesses anabolic properties and has not yet been approved for therapeutic use. Nevertheless, it is readily available on the black market. Although the World Anti-Doping Agency (WADA) has banned the use of MGF in sports, no routinely performed methods have been reported for its detection. In this work, two preparations from the black market containing an unknown MGF analogue were characterized. Mass spectrometry characterizations of unknown preparations and a reference human MGF were performed on an Orbitrap and a triple quadrupole mass spectrometers after separation by liquid chromatography. High accuracy measurements allowed protein identification from full scan MS data, and low-resolution full scan MS/MS provided further information on fragmentation. HCD scans of the analytes showed the presence of common b series product ions in the black market preparations and the human MGF reference standard, but all the y series ions starting from (y(1))(+) exhibited a difference of 1 m/z unit in nominal mass. This difference was demonstrated to be due to a C-terminal amidation of MGF. High-resolution data demonstrated that the black market products were...Continue Reading

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Citations

Jun 8, 2014·Journal of Pharmaceutical and Biomedical Analysis·Mario Thevis, Wilhelm Schänzer
Dec 4, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Mario ThevisWilhelm Schänzer
Nov 11, 2014·Expert Review of Proteomics·Mario ThevisWilhelm Schänzer
Jan 16, 2016·Drug Testing and Analysis·Lore GeldofKoen Deventer
Sep 3, 2013·Rapid Communications in Mass Spectrometry : RCM·Holly D Cox, Daniel Eichner
Dec 12, 2012·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Mar 19, 2014·Drug Testing and Analysis·Andreas KimergårdAllan Stensballe
Jul 28, 2015·Mass Spectrometry Reviews·Wilhelm Schänzer, Mario Thevis
Oct 6, 2016·International Journal of Molecular Sciences·Lore GeldofPeter Van Eeno
Dec 31, 2016·Drug Testing and Analysis·Holly D CoxDaniel Eichner

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