PMID: 9438146Jul 25, 1996Paper

Characterization and identification of EGF-receptors as an integral component of membrane structure of human endometrial stromal cells in vitro

European Journal of Medical Research
G A Singer, T Strowitzki

Abstract

The epidermal growth factor EGF is a well known mitogenic agent, elicits significant biological responses in intact animals, organs and cell cultures, and might be involved in the endometrial receptivity for successful embryo implantation. Although immunohistological and binding studies have detected EGF-binding activity in endometrial tissues, the nature of the EGF- binding site has not been fully investigated. Therefore, this study was performed to characterize and identify the nature of the endometrial stromal cell EGF-binding activity and to demonstrate EGF effects on stromal cell growth under in vitro conditions. Stimulation of cellular proliferation with EGF in various concentrations for 6 days revealed a significant increase of the proliferation rate up to 150% of that found in control subjects in a dose-dependent fashion, reaching a maximum stimulation at 10 nM EGF. Binding studies with labelled 125I-EGF confirmed the localization of the EGF-binding site in the membrane fraction of stromal cells. Furthermore, this binding activity was found to be a ligand-saturable molecular species with a dissociation constant of about 1.75 nM, and with a high specificity to EGF ligands. Affinity cross-linking studies revealed that the...Continue Reading

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