Characterization and immunological analysis of the rhesus cytomegalovirus homologue (Rh112) of the human cytomegalovirus UL83 lower matrix phosphoprotein (pp65)

The Journal of General Virology
Yujuan YuePeter A Barry

Abstract

Rhesus cytomegalovirus (RhCMV) contains two open reading frames (Rh111 and Rh112) that encode proteins homologous to the phosphoprotein 65 (pp65) of the human cytomegalovirus (HCMV) UL83 gene. As HCMV pp65 elicits protective immune responses in infected humans and represents an important vaccination target, one RhCMV homologue of HCMV pp65, pp65-2 (Rh112), was characterized and analysed for its ability to induce host immune responses. Similar to its HCMV counterpart, RhCMV pp65-2 was expressed as a late gene, localized to the nucleus within pp65-2-expressing cells and was present within infectious virions. Longitudinal and cross-sectional studies of pp65-2 immunity in naturally infected rhesus macaques showed that humoral responses to pp65-2 were elicited early during infection, but were not always sustained over time. In contrast, pp65-2-specific T-cell responses, examined by gamma interferon ELISPOT, were broadly detectable in all of the animals studied during primary infection and persisted in the vast majority of RhCMV-seropositive monkeys. Moreover, there was considerable inter-animal variability in the pattern of the immune responses to pp65-2. Together, these results demonstrated that RhCMV pp65-2 exhibited biological an...Continue Reading

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Dec 4, 2003·The Journal of General Virology·Yujuan YuePeter A Barry
Apr 24, 2004·The Journal of General Virology·Aidan DolanAndrew J Davison

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Citations

Jan 15, 2008·Medical Microbiology and Immunology·Colin Powers, Klaus Früh
Apr 1, 2014·Viruses·Jesse D Deere, Peter A Barry
Apr 3, 2014·The Journal of Clinical Investigation·Daniel MalouliKlaus Früh
Jul 28, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nicholas L PomplunEva G Rakasz
Jun 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jesse D DeereDennis J Hartigan-O'Connor
Jan 8, 2020·Viruses·Michela Perotti, Laurent Perez
Mar 7, 2020·The Journal of Infectious Diseases·Hunter K RoarkMark R Schleiss

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