Characterization and In Silico Analysis of Pregnancy-Associated Glycoprotein-1 Gene of Buffalo (Bubalus bubalis).

Genetics Research International
Jerome AAshwin Raut

Abstract

Pregnancy-Associated Glycoproteins (PAGs) are trophoblastic proteins belonging to the Aspartic proteinase family secreted by different placental cells of many mammalian species. They play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. The identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. Considering importance of these proteins, the present study was undertaken to characterize the pregnancy associated glycoprotein-1 gene of buffalo. An 1181 base pairs buffalo Pregnancy-Associated Glycoprotein PAG-1 gene was PCR amplified from the RNA obtained from the fetal cotyledons. BLAST analysis of the buffalo PAG-1 sequence retrieved a total of 20 cattle, 5 goat, and 4 sheep PAG sequences, exhibiting more than 80% similarity. Buffalo PAG-1 gene contained an uninterrupted open reading frame of 1140 base pairs encoding 380 amino acids that possess a 15 amino acid signal peptide and mature peptide of 365 amino acids. The phylogenetic study of the buffalo PAG-1 gene revealed buffalo PAG-1 is more related to cattle, goat, and sheep PAG-1 sequences. By this study characterization of buffalo PAG-1 gene and its e...Continue Reading

References

Jul 1, 1991·Biology of Reproduction·A P ZoliF Ectors
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S C XieR M Roberts
Jun 1, 1982·Biology of Reproduction·J E ButlerR J Williams
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Datasets Mentioned

BETA
EU815059
AF036952
AY509865

Methods Mentioned

BETA
electrophoresis
PCR
glycosylation

Software Mentioned

MegAlign
Primer select
DNA star
MEGA
DNASTAR
Lasergene
BLAST
SWISS MODEL
PROSITE
SAPS

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