Characterization and in vitro antioxidant activities of polysaccharides from Pleurotus ostreatus

International Journal of Biological Macromolecules
Yunxia ZhangLiangwen Chen

Abstract

Two polysaccharide fractions (PSPO-1a and PSPO-4a) were isolated from the fruiting bodies of Pleurotus ostreatus using ethanol precipitation, anion-exchange chromatography and gel permeation chromatography. Both fractions were heteropolysaccharide containing protein and uronic acid. PSPO-1a was composed of mannose, glucose, galactose, xylose and rhamnose with a molar ratio of 2.47:0.91:1.00:1.66:3.87. PSPO-4a was composed of only three monosaccharides: rhamnose, mannose and galactose with a molar ratio of 0.92:2.69:1.00. The average molecular weight of PSPO-1a and PSPO-4a determined by HPLC were estimated to be 1.8 × 10(4)Da and 1.1 × 10(6)Da respectively. The in vitro tests revealed that two polysaccharides were natural potential antioxidant. Both polysaccharides presented stronger DPPH radical and superoxide anion radical scavenging activity with increasing concentrations, but less effective on scavenging hydroxyl radical. Compared with PSPO-4a, PSPO-1a was the more effective free-radical scavenger. In conclusion, the two polysaccharides may be useful as a naturally potential antioxidant agent for application in food and medicinal fields.

References

Aug 15, 1991·Analytical Biochemistry·T M Filisetti-Cozzi, N C Carpita
Aug 6, 1998·Bioscience, Biotechnology, and Biochemistry·T YamaguchiJ Terao
Jun 20, 2006·International Immunopharmacology·Itisam SarangiTapas K Maiti
May 1, 2007·International Immunopharmacology·Mohammad-Fata MoradaliGhorban-Ali Hedjaroude
May 19, 2009·International Journal of Biological Macromolecules·Liqin SunCuihua Ma
Aug 12, 2009·Bioresource Technology·Aline Augusti BoligonMargareth Linde Athayde
Aug 25, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Somaia A NadaHanan G Zahran
Dec 31, 2010·Applied Microbiology and Biotechnology·Solomon P Wasser

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Citations

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Sep 9, 2015·Food & Function·Xiaojun Huang, Shaoping Nie
Dec 12, 2012·Journal of Separation Science·De-jun HuShao-ping Li
Jan 12, 2021·Expert Opinion on Therapeutic Patents·Martina BortolamiFabiana Pandolfi
Mar 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Siqian LiNagendra Prasad Shah
Jun 5, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Damini KothariSoo-Ki Kim

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