Characterization and in vitro assembly of tick-borne encephalitis virus C protein.

FEBS Letters
Filip KaufmanMichaela Rumlová

Abstract

Tick-borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical-chemical approaches, such as size-exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid-like structure with that of dengue C protein.

References

Jan 1, 1990·Annual Review of Microbiology·T J ChambersC M Rice
May 28, 2003·Journal of Virology·Christopher T JonesRichard J Kuhn
May 30, 2003·The EMBO Journal·Ying ZhangMichael G Rossmann
Oct 11, 2003·Science·Suchetana MukhopadhyayRichard J Kuhn
Dec 24, 2003·Protein Science : a Publication of the Protein Society·Narasimha Sreerama, Robert W Woody
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lixin MaCarol Beth Post
Apr 6, 2004·Methods in Enzymology·Narasimha Sreerama, Robert W Woody
Oct 27, 2006·Journal of Structural Biology·Ying ZhangMichael G Rossmann
Nov 18, 2011·Journal of Virology·Romana HadravováMichaela Rumlová
Dec 16, 2011·Journal of Virology·Ivana KřízováIva Pichová
Jun 15, 2012·Wiener medizinische Wochenschrift·Reinhard Kaiser
Dec 18, 2012·Nature Structural & Molecular Biology·Xiaokang ZhangZ Hong Zhou
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Dec 18, 2013·PloS One·João Miguel FreireMiguel A R B Castanho
Jul 9, 2014·The Journal of General Virology·Martin PalusDaniel Růžek
Oct 3, 2014·Journal of Virology·Karolína Strohalmová-BohmováMichaela Rumlová
May 20, 2015·World Journal of Clinical Cases·Petra Bogovic, Franc Strle
Jun 16, 2015·Scientific Reports·Tomáš BílýDaniel Růžek
Sep 28, 2015·Virology·Romana HadravováTomáš Ruml
Apr 2, 2016·Science·Devika SirohiRichard J Kuhn
Sep 27, 2016·Antiviral Research·Mariano A Garcia-BlancoChristopher Nicchitta
Nov 5, 2016·Virus Research·Edson R A OliveiraBruno A C Horta
Aug 2, 2017·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·P TabaI Steiner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.