Characterization and in vitro differentiation potential of a new human embryonic stem cell line, ReliCellhES1

Differentiation; Research in Biological Diversity
Arundhati MandalAparna Khanna

Abstract

Human embryonic stem cells (hESCs) are an exceptionally useful tool for studies of human development and represent a potential source for transplantation therapies. At present, only a limited number of hESCs lines representing a very small sample of genetic diversity of the human populations are available. Here, we report the derivation and characterization of a new hESC line, ReliCellhES1. These cells, established from the inner cell mass (ICM) on mouse embryonic feeder (MEF) layer, satisfy the criteria that characterize pluripotent hESCs: The cell line expresses high levels of cell surface markers (such as SSEA-3, SSAEA-4, TRA-1-60 and TRA-1-81), transcription factor Oct-4, alkaline phosphatase (AP) and telomerase. The cell line retains normal karyotype in long-term culture and has a distinct identity as revealed by DNA fingerprinting by short tandem repeat (STR) analysis. Further, upon examination of the in vitro differentiation potential, ReliCellhES1 was found to be capable of giving rise to dopaminergic neurons, cardiomyocytes, pancreatic islets, and hepatocyte-like cells belonging to ectoderm, mesoderm, and endoderm lineages, respectively. To our knowledge, this is the first report of a well-characterized hES cell line f...Continue Reading

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Citations

May 27, 2011·In Vitro Cellular & Developmental Biology. Animal·Punam NagvenkarDeepa Bhartiya
Dec 18, 2012·In Vitro Cellular & Developmental Biology. Animal·Arundhati MandalChandra Viswanathan
Jul 12, 2008·Stem Cells and Development·Maneesha S InamdarK VijayRaghavan
Aug 11, 2006·Genome Research·Marina BibikovaJian-Bing Fan
May 7, 2010·BMC Biotechnology·Qing Meng PiYilin Cao
Aug 22, 2006·BMC Biology·Richard JosephsonJonathan M Auerbach
May 1, 2007·Regenerative Medicine·Rajarshi PalAparna Khanna
Dec 4, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Nobuhiro ShimozawaTadashi Sankai
Aug 18, 2012·Cell Biology International·Bipasha BosePralhad Wangikar
Sep 10, 2010·Stem Cell Research·Arundhati MandalAnish Sen Majumdar
Aug 29, 2006·Human Reproduction Update·C Allegrucci, L E Young
Oct 21, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M FernándezL Calzà
Jun 10, 2010·Journal of Biomolecular Screening·Murali Krishna MamidiSatish Totey
Sep 10, 2008·Cell Biology International·Ashish MehtaAnish S Majumdar
Aug 8, 2020·The Journal of Biological Chemistry·Jeong Su ByunBaek-Soo Han

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