PMID: 6413126Jan 1, 1983Paper

Characterization and kinetics of beta-D-gluco/fuco/galactosidase from sheep liver

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
M A ChinchetruP Calvo

Abstract

This enzyme shows beta-D-glucosidase, beta-D-fucosidase and beta-D-galactosidase activities, all associated in a single peak in Sephadex G-200, DEAE-cellulose, concanavalin A-Sepharose chromatographies, and in high resolution isoelectric focusing (pI 4.56), having the optimal pH in the range 4.5-5.5. The enzyme is very stable under different conditions: (i) at pH in the range 5.5-7.0; (ii) in successive freezing-thawing cycles; (iii) at 4 degrees C; (iv) after exhaustive ultrasonic treatment. It is not stable beyond 40 degrees C, and in the presence of urea, Triton X-100, SDS or mercaptoethanol. HgCl2, KCN, Tris, maltose and the lactones were inhibitors of the enzyme. With glucose, fucose and galactose the inhibition is competitive. In addition, a transglycosylation mechanism seems to occur. The kinetic studies suggest a substrate-activation model and the presence of two primary active sites: fuco-gluco and galacto.

References

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Citations

Jan 1, 1989·The International Journal of Biochemistry·M A ChinchetruP Calvo
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A J Santa CeciliaP Calvo
Jan 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·I San-JuanP Calvo
Apr 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J CyganW L Daniel
Oct 1, 1992·Archives of Biochemistry and Biophysics·Y C ZengS Z Zhang

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