Characterization and molecular cloning of one novel C-type lectin from the venom of Taiwan habu (Trimeresurus mucrosquamatus)

Toxicon : Official Journal of the International Society on Toxinology
Yen-Shan ChenShyh-Horng Chiou

Abstract

One novel snake venom factor (termed trimecetin) was isolated and purified from the venom of Taiwan habu (Trimeresurus mucrosquamatus). The purified venom factor was shown to consist of two subunit chains linked by one disulfide bond. This two-chain factor showed high sequence homology at their N-terminal segments to some previously reported venom proteins such as alboaggregin-B isolated from Trimeresurus albolabris and agkicetin from Agkistrodon acutus. The cDNA clones corresponding to the two subunit chains, a basic chain (pI 8.97) of 133 amino acids and an acidic chain (pI 6.32) of 121 amino acids, were found to share a sequence similarity of 42.6 %. Similar to botrocetin, bitiscetin and flavocetin A characterized from other snake species, trimecetin from Taiwan habu was also shown to be a C-type lectin based on the phylogenetic and sequence comparisons of various two-chain factors from snake species of different families. The unique functional variation and evolution of trimecetin may offer some insights into the mechanism underlying the receptor recognition associated with activation or inhibition of platelet aggregation for this family of snake venom proteins.

References

Feb 20, 1976·Biochimica Et Biophysica Acta·C Ouyang, C M Teng
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·K FukudaT Morita
Jun 30, 2000·Biochemical and Biophysical Research Communications·F NiedergangM Leduc
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Jul 26, 2003·The Journal of Biological Chemistry·Sachiko KanajiThomas J Kunicki
Dec 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Thil BatuwangalaE Yvonne Jones
Jan 1, 1987·Veterinary Clinical Pathology·M A TurrentineG S Johnson
Dec 23, 2004·Journal of Thrombosis and Haemostasis : JTH·J LiuT K Gartner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Coagulation Signaling Pathways

Coagulation is the process by which a blood clot is formed. This process includes both the formation of a platelet plug as well as a cascade of clotting factors resulting in the formation of fibrin strands. Find the latest research on coagulation signaling pathways here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.