Characterization and molecular cloning of conjugation-regulating sex pheromones in homothallic Closterium

Plant & Cell Physiology
Yuki TsuchikaneHiroyuki Sekimoto

Abstract

Conjugation-regulating pheromones were analyzed in homothallic Closterium for the first time. Members of the Closterium peracerosum-strigosum-littorale complex are unicellular charophycean algae in which there are two modes of zygospore formation: heterothallism and homothallism. A homothallic strain of Closterium (designation, kodama20) forms selfing zygospores via the conjugation of two sister gametangial cells derived from one vegetative cell. Conjugation-promoting and -suppressing activities, against cells at very low (1 x 10(2) cells ml(-1)) and normal (1 x 10(4) cells ml(-1)) cell density, respectively, were detected in the medium in which cells of a normal density had been cultured. Pheromone activities decreased to 20% after incubation at 60 °C for 10  min. The release and action of the pheromones was dependent on light. The culture medium was subjected to gel filtration, and both active substances had an apparent molecular mass of 17  kDa; this was similar to that previously reported for the heterothallic sex-specific pheromone protoplast-release-inducing protein (PR-IP) Inducer. cDNAs encoding the orthologs of PR-IP Inducer were isolated from the homothallic strain. Recombinant PR-IP Inducers produced by yeast cells s...Continue Reading

References

Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Oct 29, 2003·Plant & Cell Physiology·Satoko AkatsukaTadashi Fujii
Feb 25, 2006·Science·Andrew Camilli, Bonnie L Bassler
May 17, 2006·Trends in Ecology & Evolution·Richard M McCourtKenneth G Karol

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Citations

Aug 23, 2011·Sexual Plant Reproduction·Yuki TsuchikaneHiroyuki Sekimoto
Jul 12, 2018·The New Phytologist·Yuki Tsuchikane, Hiroyuki Sekimoto
Apr 28, 2017·Proceedings. Biological Sciences·Xiao-Lan WenJan Šobotník
Jun 1, 2019·Annual Review of Microbiology·James Umen, Susana Coelho
Feb 12, 2017·Journal of Plant Research·Hiroyuki Sekimoto

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