Abstract
Calotropis procera R. Br. carboxylesterase (EC 3.1.1.1) solubilized in reversed micellar glycerol droplets containing a very small amount of water (less than 5ppm) and stabilized by a surfactant effectively catalyzed the esterification between glycerol and capric acid to produce 1-monocaprin. Reaction variables including surfactant types, organic solvent media, reaction time, G-value ([glycerol]/[capric acid]), R-value ([water]/[surfactant]), pH, temperature, and types of metal ion inhibitors on the carboxylesterase-catalyzed esterification were characterized and optimized to efficiently produce 1-monocaprin. Bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and isooctane were the most effective surfactant and organic solvent medium, respectively, for 1-monocaprin formation in reversed micelles. The optimum G- and R-values were 3.0 and 0.05, respectively, and the optimum pH and temperature were determined to be 10.0 and 60 degrees C, respectively. K(m,app.) and V(max,app.) were calculated from a Hanes-Woolf plot, and the values were 9.64 mM and 2.45 microM/min mg protein, respectively. Among various metal ions, Cu(2+) and Fe(2+) severely inhibited carboxylesterase-catalyzed esterification activity (less than 6.0% of relative activi...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Feb 24, 1988·Biochimica Et Biophysica Acta·P L LuisiB H Robinson
Jun 15, 1984·Science·A Zaks, A M Klibanov
Oct 26, 1995·Biochimica Et Biophysica Acta·G D ReesG R Stephenson
Oct 9, 1999·Journal of Pharmaceutical Sciences·T KristmundsdóttirH Thormar
Oct 9, 1999·Journal of Pharmaceutical Sciences·S DöppenschmittP Langguth
Apr 4, 2000·Journal of Medical Virology·J NeytsH Thormar
Jun 23, 2000·Enzyme and Microbial Technology·X GaoaK Zhang
Feb 22, 2002·Biotechnology and Bioengineering·N I Gerhardt, S R Dungan
Jan 5, 2006·Applied and Environmental Microbiology·Halldor ThormarGudmundur Bergsson
Dec 5, 1991·Biotechnology and Bioengineering·P S ChangJ J Kim
Aug 20, 1991·Biotechnology and Bioengineering·D G Hayes, E Gulari
Aug 30, 2008·The Journal of Physical Chemistry. B·Dagmar BilanicováMaura Monduzzi
Jan 1, 1998·Bioscience, Biotechnology, and Biochemistry·M ShimaR Matsuno
Citations
Dec 18, 2015·Enzyme and Microbial Technology·Kyung-Min ParkPahn-Shick Chang
Apr 7, 2015·Food Chemistry·Chang Woo KwonPahn-Shick Chang
Feb 28, 2015·Food Chemistry·Sung-Chul HongPahn-Shick Chang
Nov 14, 2018·Biotechnology Progress·Jun-Young ParkPahn-Shick Chang
Aug 30, 2013·Journal of Agricultural and Food Chemistry·Kyung Min ParkPahn-Shick Chang