Characterization and partial purification of a bacteriocin produced by Leuconostoc carnosum LA44A

International Journal of Food Microbiology
R L van LaackW H Holzapfel

Abstract

Twenty Leuconostoc strains isolated from vacuum packaged Vienna-type sausages were screened for antagonistic activity against various Gram-positive organisms (including Listeria spp.). One of the three strains exhibiting inhibitory activity was chosen for further investigation. This strain was identified as Leuc. carnosum and the inhibitory substance produced was named carnosin. Carnosin was inactivated by trypsin but not by catalase or other non-proteolytic enzymes tested. Carnosin retained activity after heating at 100 degrees C for 20 min, whereas heating at 121 degrees C for 15 min resulted in complete loss of activity. Carnosin was active at pH values ranging from 2 to 9. Carnosin activity was not detectable until cells were in the late log-phase of growth. At low temperatures (4 degrees C), higher cell densities were required before carnosin activity could be detected. Carnosin was active against various lactic acid bacteria, Enterococcus faecalis and Enterococcus faecium and against Listeria spp. Difficulties in purification were reduced by growing Leuc. carnosum in a modified MRS medium, having 50% of the normal peptone concentration and no Tween or meat extract. Sodium dodecyl sulphate polyacrylamide gel electrophoresi...Continue Reading

References

Feb 1, 1991·The Journal of Applied Bacteriology·J W Hastings, M E Stiles
Sep 1, 1990·The Journal of Applied Bacteriology·C Ahn, M E Stiles
Mar 1, 1988·Biochimie·T R Klaenhammer
Jan 1, 1970·European Journal of Biochemistry·J De LeyA Reynaerts
Sep 1, 1984·Antimicrobial Agents and Chemotherapy·S F Barefoot, T R Klaenhammer
Jan 1, 1983·Systematic and Applied Microbiology·V A HussK H Schleifer

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Citations

Jan 1, 1994·Journal of Basic Microbiology·M A PapathanasopoulosA von Holy
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Archana KumariMustafa Akçelik
Nov 26, 1993·International Journal of Food Microbiology·U SchillingerW H Holzapfel
Jan 1, 1995·International Journal of Food Microbiology·W H HolzapfelU Schillinger
Apr 1, 1995·International Journal of Food Microbiology·F VillaniS Coppola
Dec 1, 1996·International Journal of Food Microbiology·E ParenteA Ricciardi
Jan 1, 1997·International Journal of Food Microbiology·V Carolissen-MackayJ W Hastings
May 3, 2000·Journal of Applied Microbiology·W M ZhuD Q Wu
Aug 1, 1996·Letters in Applied Microbiology·A M Revol-JunellesG Lefebvre
Feb 8, 2006·Applied and Environmental Microbiology·Tina HornbaekBirgitte Bjørn Budde
Jul 13, 2004·The Journal of General and Applied Microbiology·Marilingappa Jamuna, Kadirvelu Jeevaratnam
Jun 8, 2014·Probiotics and Antimicrobial Proteins·Aline Teodoro de PaulaAna Lúcia Barretto Penna
Jul 15, 2004·Journal of Industrial Microbiology & Biotechnology·Svetoslav D Todorov, Leon M T Dicks
Sep 1, 1994·Journal of Dairy Science·M E Stiles
Jan 29, 2021·Frontiers in Microbiology·Francesco CandeliereMaddalena Rossi
Jul 1, 1993·Applied and Environmental Microbiology·G R Siragusa, C N Cutter

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