Characterization and partial purification of bovine alpha-lactalbumin and beta-casein produced in milk of transgenic mice

Journal of Dairy Science
S Y JengR Jimenez-Flores

Abstract

Bovine alpha-lactalbumin (alpha-LA) and bovine beta-casein (beta-CN), from milk from transgenic mice were characterized and partially purified using electrophoretic, immunoblotting, and chromatographic methods. The transgenically expressed bovine milk proteins were identified using PAGE or by a combination of preparative isoelectrofocusing followed by Western immunoblotting. The heterologous bovine alpha-IA and bovine beta-CN had molecular masses that were identical to those of those of the native proteins. The estimated expression of the proteins was 1.0 mg/ml of milk for alpha-LA and 3.0 mg/ml for beta-CN. The calcium binding of bovine alpha-LA suggested that the protein produced in murine milk has the same electrophoretic shift as native bovine alpha-LA after the removal of calcium. Nitrogen-linked glycosylation of native and murine synthesized bovine alpha-LA was identified by peptide-N-glycosidase F treatment, and the N-terminal amino acid sequence of HPLC-purified bovine alpha-LA from mouse milk was confirmed to be identical to native bovine alpha-LA. In addition, the phosphorylation of the bovine beta-CN expressed in the milk of transgenic mice was the same as that of native bovine beta-CN, as determined by phosphatase d...Continue Reading

References

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Citations

Nov 5, 2004·Science in China. Series C, Life Sciences·Siguo LiuWeide Lao
Jul 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·R PhilipL O. Vodkin
Nov 13, 2004·The Journal of Nutritional Biochemistry·P A PrietoB Kelder
Jun 15, 2021·Journal of Mammary Gland Biology and Neoplasia·Anna SadovnikovaRussell C Hovey

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