PMID: 11921402Mar 29, 2002Paper

Characterization and peroxidase activity of a myoglobin mutant containing a distal arginine

Chembiochem : a European Journal of Chemical Biology
C RedaelliLucia Banci

Abstract

The spectroscopic, conformational, and reactivity characteristics of the T67R variant of sperm whale myoglobin have been studied to assess the effects of introducing an arginine residue into the distal side of this protein, as occurs in the active site of heme peroxidases. The overall circular dichroism (CD) and NMR spectroscopic properties of various derivatives of the protein are little affected by the mutation. The mutant contains a high-spin ferric ion with a water molecule as the sixth ligand, which exhibits slightly enhanced acidity (pK(a)=8.43+/-0.03) with respect to the corresponding derivative of wild-type myoglobin (pK(a)=8.60+/-0.04). The presence of the distal arginine increases the affinity of the Fe(III) center for azide (K=(6.0+/-0.5)x10(4) M(-1)) and decreases that for imidazole (K=12.0+/-0.2 M(-1)), with respect to the wild-type protein (K=(5.0+/-0.1)x10(4) and 24.7+/-0.7 M(-1), respectively). The peroxidase activity of T67R and wild-type myoglobins has been studied with a group of phenolic substrates related to tyrosine. The mutant exhibits an increased rate of reaction with hydrogen peroxide (k=1550+/-10 versus 760+/-10 M(-1) x s(-1)) and a generally increased peroxidase activity with respect to wild-type myo...Continue Reading

Citations

Nov 27, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Raffaella RonconeLuigi Casella
Sep 29, 2011·Biochemistry·Edward L D'AntonioStefan Franzen
Nov 22, 2012·Inorganic Chemistry·Yao ChenShengqian Ma
Apr 30, 2010·Physical Chemistry Chemical Physics : PCCP·Nora BergmannEmad F Aziz
Dec 26, 2008·IUBMB Life·Elisabetta De MarinisPaolo Ascenzi
Apr 2, 2008·IUBMB Life·Enrico MonzaniLuigi Casella
Aug 9, 2008·Chemistry : a European Journal·Stefania NicolisLuigi Casella
Aug 8, 2015·Accounts of Chemical Research·Chandradeep GhoshSomdatta Ghosh Dey
Aug 25, 2015·Biochimica Et Biophysica Acta·Parisa Hosseinzadeh, Yi Lu
Jun 20, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gianantonio BattistuzziMarco Sola
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Nov 21, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Francesca BellezzaSilvia Tacchi
Aug 30, 2012·The Journal of Physical Chemistry. B·Junjie ZhaoStefan Franzen
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Jun 30, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Francesca BellezzaStefania Nicolis

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