Characterization and phylogenetic analysis of Bovine viral diarrhea virus in brain tissues from nonambulatory (downer) cattle in Korea

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
J K OemO S Lee

Abstract

Between August 2008 and May 2009, 386 brain and serum samples from adult cattle (2-7 years old) showing a variety of clinical signs of downer cow syndrome were received by the National Veterinary Research and Quarantine Service. All brain samples were tested for the presence of Bovine viral diarrhea virus (BVDV) by reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and antigen capture ELISA (Ag-ELISA). The BVDV nucleic acid was detected in 54 of 386 (15.5%) brain samples tested by RT-PCR. Positive results were detected in 14 (3.67%) and 13 (3.4%) of samples tested by IHC and Ag-ELISA, respectively. Both BVDV nucleic acid and antigen were detected in 11 cattle (2.9%) by all 3 diagnostic tests; however, antibodies against BVDV were not detected in these 11 cattle. A molecular classification of the identified viral strains (n = 40) was also carried out. Neighbor-joining phylogenetic analysis revealed that most of the identified viruses belonged to BVDV genotype 1a (n = 10), 1b (n = 16), and 2a (n = 8). The remaining strains were subtypes 1c (n = 1), 1n (n = 4), and 1m (n = 1). Interestingly, most of the BVDV-1b strains (n = 9) identified in brain samples were confirmed by all 3 diagnostic tests. ...Continue Reading

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Citations

Apr 28, 2010·Archives of Pharmacal Research·Eun-Ju YangKyung-Sik Song
Jul 24, 2012·Biologicals : Journal of the International Association of Biological Standardization·M Daniel Givens, M Shonda Marley
Aug 28, 2015·The Journal of Veterinary Medical Science·Kaizhao ZhangZhangyong Ning
Oct 25, 2014·The Journal of Veterinary Medical Science·Yeon-Hee KimSe Chang Park
Mar 18, 2017·The Journal of Veterinary Medical Science·Suryo Purnomo EdiHiroaki Shirafuji
Aug 9, 2018·BMC Veterinary Research·Du-Gyeong HanKyoung-Seong Choi
Apr 8, 2019·Tropical Animal Health and Production·Ji-Hyoung Ryu, Kyoung-Seong Choi

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Methods Mentioned

BETA
chip

Software Mentioned

BioEdit
Molecular Evolutionary Genetics Analysis ( MEGA )
Megna

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