Characterization and phylogenetic epitope mapping of CD38 ADPR cyclase in the cynomolgus macaque

BMC Immunology
Enza FerreroFabio Malavasi

Abstract

The CD38 transmembrane glycoprotein is an ADP-ribosyl cyclase that moonlights as a receptor in cells of the immune system. Both functions are independently implicated in numerous areas related to human health. This study originated from an inherent interest in studying CD38 in the cynomolgus monkey (Macaca fascicularis), a species closely related to humans that also represents a cogent animal model for the biomedical analysis of CD38. A cDNA was isolated from cynomolgus macaque peripheral blood leukocytes and is predicted to encode a type II membrane protein of 301 amino acids with 92% identity to human CD38. Both RT-PCR-mediated cDNA cloning and genomic DNA PCR surveying were possible with heterologous human CD38 primers, demonstrating the striking conservation of CD38 in these primates. Transfection of the cDNA coincided with: (i) surface expression of cynomolgus macaque CD38 by immunofluorescence; (ii) detection of approximately 42 and 84 kDa proteins by Western blot and (iii) the appearance of ecto-enzymatic activity. Monoclonal antibodies were raised against the cynomolgus CD38 ectodomain and were either species-specific or cross-reactive with human CD38, in which case they were directed against a common disulfide-requirin...Continue Reading

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Citations

Sep 6, 2007·Journal of Cellular Biochemistry·Oriana TrubianiRoberto Di Primio
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Robert C Ladner
Apr 1, 2021·Physiological Reviews·Alberto L HorensteinFabio Malavasi

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Datasets Mentioned

BETA
AY555148

Methods Mentioned

BETA
glycosylation
PCR
confocal microscopy
FACS

Software Mentioned

CellQuest
RasWin Molecular Graphics
TRANSFAC
FluoView

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