Characterization and potential use of a Cryptosporidium parvum virus (CPV) antigen for detecting C. parvum oocysts

Journal of Microbiological Methods
K E KnielM C Jenkins

Abstract

The purpose of this study was to characterize the viral symbiont (CPV) of Cryptosporidium parvum sporozoites and evaluate the CPV capsid protein (CPV40) as a target for sensitive detection of the parasite. Recombinant CPV40 was produced in Escherichia coli, purified by affinity chromatography, and used to prepare polyclonal rabbit sera specific for the viral capsid protein. Anti-rCPV40 recognized a 40 kDa and a 30 kDa protein in C. parvum oocysts and appeared to localize to the apical end of the parasite. Anti-rCPV40 serum was capable of detecting as few as 1 C. parvum oocyst in a dot blot assay, the sensitivity being at least 1000-fold greater than sera reactive with total native C. parvum oocyst protein or specific for the 41 kDa oocyst surface antigen. Water samples were seeded with C. parvum oocysts and incubated at 4, 20, or 25 degrees C for greater than 3 months to determine if CPV levels were correlated with oocyst infectivity. Samples were removed monthly and subjected to mouse and cell culture infectivity, as well as PCR analysis for infectivity and viral particle presence. While sporozoite infectivity declined by more than 75% after 1 month at 25 degrees C, the CPV signal was similar to that of control samples at 4 de...Continue Reading

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Citations

Oct 27, 2009·Archives of Virology·Max L NibertSaid A Ghabrial
Jan 15, 2014·BMC Infectious Diseases·Bas B Oude MunninkLia van der Hoek
May 30, 2006·The Journal of Parasitology·Mark C JenkinsRonald Fayer
Feb 21, 2007·Microbiology and Immunology·Yoshihito FujinamiJiro Yasuda
Sep 30, 2010·Experimental Parasitology·Jorge FragaAyme Fernández-Calienes
Apr 1, 2008·The Science of the Total Environment·Roberto ReinosoEloy Bécares
Dec 22, 2007·International Journal for Parasitology·M C JenkinsR Fayer
Aug 2, 2005·Water Research·Pamela L Young, Simeon J Komisar
May 17, 2017·The Korean Journal of Parasitology·Ding HeZhang Xichen
Jan 15, 2013·Parasitology Research·Jennifer A ZambriskiHussni O Mohammed

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