Characterization and primary functional analysis of phenylalanine ammonia-lyase gene from Phyllostachys edulis.

Plant Cell Reports
Z M GaoQ Liu

Abstract

Phenylalanine ammonia-lyase (PAL) catalyzes the first reaction in phenylpropanoid pathway leading to the production of phenolic compounds with a wide range of biological functions. The cDNA encoding PAL was isolated from Phyllostachys edulis by reverse transcription-polymerase chain reaction (RT-PCR) and by 5' and 3' rapid amplification of cDNA ends, and was designated as PePAL. The full length of PePAL was 2,503 bp which contained an open reading frame (ORF) encoding a peptide of 701 amino acids, with a theoretic isoelectric point of 6.49 and a calculated molecular mass of 75.7 kDa. PePAL was heterologously expressed in Escherichia coli and the recombinant proteins exhibited both PAL and tyrosine ammonia-lyase (TAL) activities. The optimum temperature and pH of the recombinant PePAL were 50 °C and 8.5-9.0, respectively. The K (m) and V (max) values for L-phenylalanine was calculated as 422 μM and 45.9 nM s⁻¹, while for L-tyrosine were 78 μM and 7.09 nM s⁻¹, respectively. Tissue-specific expression assay showed that PePAL expressed highest in stem and sheath, followed by leaf, and lowest in root. Though the accumulation of PePAL proteins was observed in all these four organs by Western blotting, the highest was detected in leaf...Continue Reading

References

Jan 1, 1995·Plant Molecular Biology·L A WannerK R Davis
Jan 1, 1997·Plant Physiology·J RöslerJ Schmid
Jul 1, 1995·The Plant Cell·R. A. Dixon, N. L. Paiva
Apr 3, 2003·Journal of Agricultural and Food Chemistry·Mercedes E ChamanVictor H Argandoña
Apr 11, 2003·Biochimica Et Biophysica Acta·János Rétey
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Jason MacDonald, Godwin B D'Cunha
Sep 20, 2007·Science in China. Series C, Life Sciences·YiJie GuiLongJiang Fan
Dec 2, 2008·Biological & Pharmaceutical Bulletin·Taketo OkadaSetsuko Sekita
Apr 1, 2010·Molecular Biology Reports·Lu-Sheng HsiehPing-Du Lee

❮ Previous
Next ❯

Citations

May 31, 2013·Biotechnology and Applied Biochemistry·Olga O BabichAlexander Yu Prosekov
Apr 6, 2013·Current Opinion in Chemical Biology·Matthew M HeberlingDick B Janssen
Oct 21, 2016·Functional & Integrative Genomics·SongTao JiuXiaoMin Wang
Jul 28, 2020·Frontiers in Plant Science·Daniel B Sulis, Jack P Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.